2G79

Crystal Structure of the R132K:Y134F Mutant of Cellular Retinoic Acid Binding Protein Type II in Complex with All-Trans-Retinal at 1.69 Angstroms Resolution

2G79 の概要

• エントリーDOI: 10.2210/pdb2g79/pdb
• 関連するPDBエントリー: 2G78 2G7A 2G7B
• 分子名称: Cellular retinoic acid-binding protein 2, SODIUM ION, SULFATE ION, ... (5 entities in total)
• 機能のキーワード: crabpii, retinoic acid, retinoids, beta barrel, high resolution, retinal, transport protein
• 由来する生物種: Homo sapiens (human)
• 細胞内の位置: Cytoplasm: P29373
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 16083.32

構造登録者

Vaezeslami, S.,Geiger, J.H. (登録日: 2006-02-27, 公開日: 2007-04-17, 最終更新日: 2024-02-14)

主引用文献

Vasileiou, C.,Vaezeslami, S.,Crist, R.M.,Rabago-Smith, M.,Geiger, J.H.,Borhan, B.
Protein design: reengineering cellular retinoic acid binding protein II into a rhodopsin protein mimic.
J.Am.Chem.Soc., 129:6140-6148, 2007

PubMed Abstract: Rational redesign of the binding pocket of Cellular Retinoic Acid Binding Protein II (CRABPII) has provided a mutant that can bind retinal as a protonated Schiff base, mimicking the binding observed in rhodopsin. The reengineering was accomplished through a series of choreographed manipulations to ultimately orient the reactive species (the epsilon-amino group of Lys132 and the carbonyl of retinal) in the proper geometry for imine formation. The guiding principle was to achieve the appropriate Bürgi-Dunitz trajectory for the reaction to ensue. Through crystallographic analysis of protein mutants incapable of forming the requisite Schiff base, a highly ordered water molecule was identified as a key culprit in orienting retinal in a nonconstructive manner. Removal of the ordered water, along with placing reinforcing mutations to favor the desired orientation of retinal, led to a triple mutant CRABPII protein capable of nanomolar binding of retinal as a protonated Schiff base. The high-resolution crystal structure of all-trans-retinal bound to the CRABPII triple mutant (1.2 A resolution) unequivocally illustrates the imine formed between retinal and the protein.

PubMed: 17447762
DOI: 10.1021/ja067546r

実験手法

X-RAY DIFFRACTION (1.69 Å)