2G5B

Crystal Structure of the anti-Bax monoclonal antibody 6A7 and a Bax peptide.

2G5B の概要

• エントリーDOI: 10.2210/pdb2g5b/pdb
• 分子名称: 6A7 Fab Light Chain, 6A7 Fab Heavy Chain, Bax Peptide, ... (8 entities in total)
• 機能のキーワード: 6a7, bax, fab, antibody, fab-peptide complex, apoptosis
• 由来する生物種: Mus musculus (house mouse); 詳細
• タンパク質・核酸の鎖数: 12
• 化学式量合計: 197961.31

構造登録者

Peyerl, F.W.,Dai, S.,Murphy, G.A.,Marrack, P.,Kappler, J.W. (登録日: 2006-02-22, 公開日: 2006-07-25, 最終更新日: 2024-11-06)

主引用文献

Peyerl, F.W.,Dai, S.,Murphy, G.A.,Crawford, F.,White, J.,Marrack, P.,Kappler, J.W.
Elucidation of some Bax conformational changes through crystallization of an antibody-peptide complex.
Cell Death Differ., 14:447-452, 2007

PubMed Abstract: The Bcl-2 family member Bax plays a critical role in apoptosis. In healthy resting cells, Bax resides in the cytoplasm and loosely attached to the mitochondrial membrane. Apoptotic stimuli induce Bax activation, which is characterized by translocation and multimerization on the mitochondrial membrane surface resulting in exposure of an amino terminal epitope recognized by the monoclonal antibody 6A7. To understand the structural changes that occur during Bax activation, we determined the crystal structure of a Bax peptide bound to the 6A7 Fab fragment to a resolution of 2.3 A. The structure reveals the conformation of the 6A7 peptide epitope on Bax in the activated form and elucidates the extensive structural changes that Bax must undergo during the conversion from its native to its activated conformation.

PubMed: 16946732
DOI: 10.1038/sj.cdd.4402025

実験手法

X-RAY DIFFRACTION (2.3 Å)