2G1J

Crystal structure of Mycobacterium tuberculosis Shikimate Kinase at 2.0 angstrom resolution

Summary for 2G1J

• Entry DOI: 10.2210/pdb2g1j/pdb
• Related: 1L4U 1L4Y 1U8A 1WE2 1ZYU
• Descriptor: Shikimate kinase, SULFATE ION (3 entities in total)
• Functional Keywords: shikimate pathway, shikimate kinase, ternary complex, drug design, transferase
• Biological source: Mycobacterium tuberculosis
• Cellular location: Cytoplasm (Probable): P0A4Z2
• Total number of polymer chains: 2
• Total formula weight: 37512.89

Authors

Gan, J.,Gu, Y.,Li, Y.,Yan, H.,Ji, X. (deposition date: 2006-02-14, release date: 2006-07-18, Last modification date: 2023-08-30)

Primary citation

Gan, J.,Gu, Y.,Li, Y.,Yan, H.,Ji, X.
Crystal Structure of Mycobacterium tuberculosis Shikimate Kinase in Complex with Shikimic Acid and an ATP Analogue.
Biochemistry, 45:8539-8545, 2006

PubMed Abstract: Shikimate kinase (SK) and other enzymes in the shikimate pathway are potential targets for developing nontoxic antimicrobial agents, herbicides, and antiparasite drugs, because the pathway is essential in microorganisms, plants, and parasites but absent from mammals. SK catalyzes the reaction of phosphoryl transfer from ATP to shikimic acid (SA). Since 2002, a total of 11 SK structures have been reported, but none contains either the two substrate (SA and ATP) or the two product (SA-phosphate and ADP) molecules. Here, we present three crystal structures of SK from Mycobacterium tuberculosis (MtSK), including apo-MtSK, a binary complex MtSK x SA, and the ternary complex of MtSK with SA and an ATP analogue, AMPPCP. The structures of apo-MtSK and MtSK x AMPPCP x SA make it possible to elucidate the conformational changes of MtSK upon the binding of both substrates; the structure of MtSK x AMPPCP x SA reveals interactions between the protein and gamma-phosphate which indicate dynamic roles of catalytic residues Lys15 and Arg117.

PubMed: 16834327
DOI: 10.1021/bi0606290

Experimental method

X-RAY DIFFRACTION (2 Å)