2FZ2

Structure of Turnip Yellow Mosaic Virus at 100 K

Summary for 2FZ2

• Entry DOI: 10.2210/pdb2fz2/pdb
• Related: 1auy 1w39 2FZ1
• Descriptor: 5'-R(*CP*CP*C)-3', Coat protein (2 entities in total)
• Functional Keywords: plant virus, coat protein, capsid protein, tymoviruses, tymv, rna, icosahedral virus, virus-rna complex, virus/rna
• Biological source: Turnip yellow mosaic virus
• Cellular location: Virion (Potential): P20125
• Total number of polymer chains: 4
• Total formula weight: 61492.44

Authors

Larson, S.B.,Lucas, R.W.,McPherson, A. (deposition date: 2006-02-08, release date: 2006-02-21, Last modification date: 2023-08-30)

Primary citation

Larson, S.B.,Lucas, R.W.,Greenwood, A.,McPherson, A.
The RNA of turnip yellow mosaic virus exhibits icosahedral order.
Virology, 334:245-254, 2005

PubMed Abstract: Difference electron density maps, based on structure factor amplitudes and experimental phases from crystals of wild-type turnip yellow mosaic virus and those of empty capsids prepared by freeze-thawing, show a large portion of the encapsidated RNA to have an icosahedral distribution. Four unique segments of base-paired, double-helical RNA, one to two turns in length, lie between 33-A and 101-A radius and are organized about either 2-fold or 5-fold icosahedral axes. In addition, single-stranded loops of RNA invade the pentameric and hexameric capsomeres where they contact the interior capsid surface. The remaining RNA, not seen in electron density maps, must serve as connecting links between these secondary structural elements and is likely icosahedrally disordered. The distribution of RNA observed crystallographically appears to be in agreement with models based on biochemical data and secondary structural analyses.

PubMed: 15780874
DOI: 10.1016/j.virol.2005.01.036

Experimental method

X-RAY DIFFRACTION (2.9 Å)