2FYS

Crystal structure of Erk2 complex with KIM peptide derived from MKP3

2FYS の概要

• エントリーDOI: 10.2210/pdb2fys/pdb
• 分子名称: Mitogen-activated protein kinase 1, Dual specificity protein phosphatase 6 (3 entities in total)
• 機能のキーワード: map kinase, mkp3, kim, transferase
• 由来する生物種: Rattus norvegicus (Norway rat); 詳細
• 細胞内の位置: Cytoplasm: Q64346
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 88197.71

構造登録者

Liu, S.,Sun, J.P.,Zhou, B.,Zhang, Z.Y. (登録日: 2006-02-08, 公開日: 2006-04-11, 最終更新日: 2023-08-30)

主引用文献

Liu, S.,Sun, J.P.,Zhou, B.,Zhang, Z.Y.
Structural basis of docking interactions between ERK2 and MAP kinase phosphatase 3
Proc.Natl.Acad.Sci.Usa, 103:5326-5331, 2006

PubMed Abstract: Mitogen-activated protein (MAP) kinases are central components of signal transduction pathways for cell proliferation, stress responses, and differentiation. Signaling efficiency and specificity are modulated in large part by docking interactions between individual MAP kinase and the kinase interaction motif (KIM), (R/K)(2-3)-X(1-6)-Phi(A)-X-Phi(B), in its cognate kinases, phosphatases, scaffolding proteins, and substrates. We have determined the crystal structure of extracellular signal-regulated protein kinase 2 bound to the KIM peptide from MAP kinase phosphatase 3, an extracellular signal-regulated protein kinase 2-specific phosphatase. The structure reveals that the KIM docking site, situated in a noncatalytic region opposite of the kinase catalytic pocket, is comprised of a highly acidic patch and a hydrophobic groove, which engage the basic and Phi(A)-X-Phi(B) residues, respectively, in the KIM sequence. The specific docking interactions observed in the structure consolidate all known biochemical data. In addition, structural comparison indicates that the KIM docking site is conserved in all MAP kinases. The results establish a structural model for understanding how MAP kinases interact with their regulators and substrates and provide new insights into how MAP kinase docking specificity can be achieved.

PubMed: 16567630
DOI: 10.1073/pnas.0510506103

実験手法

X-RAY DIFFRACTION (2.5 Å)