2FXT

Crystal Structure of Yeast Tim44

2FXT の概要

• エントリーDOI: 10.2210/pdb2fxt/pdb
• 分子名称: Import inner membrane translocase subunit TIM44 (1 entity in total)
• 機能のキーワード: mitochondrial translocase, protein transport
• 由来する生物種: Saccharomyces cerevisiae (baker's yeast)
• 細胞内の位置: Mitochondrion inner membrane ; Peripheral membrane protein : Q01852
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 22018.16

構造登録者

Josyula, R.,Sha, B. (登録日: 2006-02-06, 公開日: 2007-02-06, 最終更新日: 2024-02-14)

主引用文献

Josyula, R.,Jin, Z.,Fu, Z.,Sha, B.
Crystal Structure of Yeast Mitochondrial Peripheral Membrane Protein Tim44p C-terminal Domain.
J.Mol.Biol., 359:798-804, 2006

PubMed Abstract: The protein transports from the cell cytosol to the mitochondria matrix are carried out by the translocase of the outer membrane (TOM) complex and the translocase of the inner membrane (TIM) complexes. Tim44p is an essential mitochondrial peripheral membrane protein and a major component of TIM23 translocon. Tim44p can tightly associate with the inner mitochondrial membrane. To investigate the mechanism by which Tim44p functions in the TIM23 translocon to deliver the mitochondrial protein precursors, we have determined the crystal structure of the yeast Tim44p C-terminal domain to 3.2A resolution using the MAD method. The Tim44p C-terminal domain forms a monomer in the crystal structure and contains six alpha-helices and four antiparallel beta-strands. A large hydrophobic pocket was identified on the Tim44p structure surface. The N-terminal helix A1 is positively charged and the helix A1 protrudes out from the Tim44p main body.

PubMed: 16647716
DOI: 10.1016/j.jmb.2006.04.020

実験手法

X-RAY DIFFRACTION (3.2 Å)