2FXL

Urate oxidase from aspergillus flavus complexed with allantoin

2FXL の概要

• エントリーDOI: 10.2210/pdb2fxl/pdb
• 関連するPDBエントリー: 1R4S 1R4U 1R51 1R56
• 分子名称: Uricase, 1-(2,5-DIOXO-2,5-DIHYDRO-1H-IMIDAZOL-4-YL)UREA (3 entities in total)
• 機能のキーワード: oxidoreductase, uric acid degradation, dimeric barrel, tunnel-shaped protein, allantoin
• 由来する生物種: Aspergillus flavus
• 細胞内の位置: Peroxisome: Q00511
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 34355.69

構造登録者

Gabison, L.,Chiadmi, M.,Colloc'h, N.,Prange, T. (登録日: 2006-02-06, 公開日: 2006-05-23, 最終更新日: 2023-08-30)

主引用文献

Gabison, L.,Chiadmi, M.,Colloc'h, N.,Castro, B.,El Hajji, M.,Prange, T.
Recapture of [S]-allantoin, the product of the two-step degradation of uric acid, by urate oxidase.
Febs Lett., 580:2087-2091, 2006

PubMed Abstract: Urate oxidase from Aspergillus flavus catalyzes the degradation of uric acid to [S]-allantoin through 5-hydroxyisourate as a metastable intermediate. The second degradation step is thought either catalyzed by another specific enzyme, or spontaneous. The structure of the enzyme was known at high resolution by X-ray diffraction of I222 crystals complexed with a purine-type inhibitor (8-azaxanthin). Analyzing the X-ray structure of urate oxidase treated with an excess of urate, the natural substrate, shows unexpectedly that the active site recaptures [S]-allantoin from the racemic end product of a second degradation step.

PubMed: 16545381
DOI: 10.1016/j.febslet.2006.03.007

実験手法

X-RAY DIFFRACTION (1.76 Å)