2FX3

Crystal Structure Determination of E. coli Elongation Factor, Tu using a Twinned Data Set

2FX3 の概要

• エントリーDOI: 10.2210/pdb2fx3/pdb
• 分子名称: Elongation factor Tu, MAGNESIUM ION, GUANOSINE-5'-DIPHOSPHATE, ... (4 entities in total)
• 機能のキーワード: ef-tu, merohedral twinning, translation
• 由来する生物種: Escherichia coli
• 細胞内の位置: Cytoplasm: Q83JC4
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 43676.78

構造登録者

Heffron, S.E.,Moeller, R.,Jurnak, F. (登録日: 2006-02-03, 公開日: 2006-03-28, 最終更新日: 2023-08-30)

主引用文献

Heffron, S.E.,Moeller, R.,Jurnak, F.
Solving the structure of Escherichia coli elongation factor Tu using a twinned data set.
Acta Crystallogr.,Sect.D, 62:433-438, 2006

PubMed Abstract: Escherichia coli elongation factor Tu-GDP (EF-Tu-GDP) was crystallized in the presence of novel inhibitors. The only crystals which could be grown were epitaxially as well as merohedrally twinned, highly mosaic and diffracted to a resolution of 3.4 A in space group P3(1)21, with unit-cell parameters a = b = 69.55, c = 169.44 A, alpha = beta = 90, gamma = 120 degrees . To determine whether an inhibitor was present in the crystal, a poor-quality X-ray diffraction data set had to be processed. The three-dimensional structure was ultimately solved and the original question answered. The results also reveal a new type of dimer packing for EF-Tu-GDP.

PubMed: 16552145
DOI: 10.1107/S0907444906004021

実験手法

X-RAY DIFFRACTION (3.4 Å)