2FWM

Crystal Structure of E. coli EntA, a 2,3-dihydrodihydroxy benzoate dehydrogenase

2FWM の概要

• エントリーDOI: 10.2210/pdb2fwm/pdb
• 分子名称: 2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase (2 entities in total)
• 機能のキーワード: enterobactin, rossmann fold, chorismate metabolism, short-chain oxidoreductase, tetramer, oxidoreductase
• 由来する生物種: Escherichia coli
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 26466.78

構造登録者

Gulick, A.M.,Duax, W.L. (登録日: 2006-02-02, 公開日: 2006-06-27, 最終更新日: 2024-02-14)

主引用文献

Sundlov, J.A.,Garringer, J.A.,Carney, J.M.,Reger, A.S.,Drake, E.J.,Duax, W.L.,Gulick, A.M.
Determination of the crystal structure of EntA, a 2,3-dihydro-2,3-dihydroxybenzoic acid dehydrogenase from Escherichia coli.
Acta Crystallogr.,Sect.D, 62:734-740, 2006

PubMed Abstract: The Escherichia coli enterobactin synthetic cluster is composed of six proteins, EntA-EntF, that form the enterobactin molecule from three serine molecules and three molecules of 2,3-dihydroxybenzoic acid (DHB). EntC, EntB and EntA catalyze the three-step synthesis of DHB from chorismate. EntA is a member of the short-chain oxidoreductase (SCOR) family of proteins and catalyzes the final step in DHB synthesis, the NAD+-dependent oxidation of 2,3-dihydro-2,3-dihydroxybenzoic acid to DHB. The structure of EntA has been determined by multi-wavelength anomalous dispersion methods. Here, the 2.0 A crystal structure of EntA in the unliganded form is presented. Analysis of the structure in light of recent structural and bioinformatic analysis of other members of the SCOR family provides insight into the residues involved in cofactor and substrate binding.

PubMed: 16790929
DOI: 10.1107/S0907444906015824

実験手法

X-RAY DIFFRACTION (2 Å)