2FQ3

Structure and function of the SWIRM domain, a conserved protein module found in chromatin regulatory complexes

2FQ3 の概要

• エントリーDOI: 10.2210/pdb2fq3/pdb
• 分子名称: Transcription regulatory protein SWI3 (2 entities in total)
• 機能のキーワード: four-helix bundle, transcription
• 由来する生物種: Saccharomyces cerevisiae (baker's yeast)
• 細胞内の位置: Nucleus: P32591
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 12382.06

構造登録者

Da, G.,Lenkart, J.,Zhao, K.,Shiekhattar, R.,Cairns, B.R.,Marmorstein, R. (登録日: 2006-01-17, 公開日: 2006-02-07, 最終更新日: 2024-02-14)

主引用文献

Da, G.,Lenkart, J.,Zhao, K.,Shiekhattar, R.,Cairns, B.R.,Marmorstein, R.
Structure and function of the SWIRM domain, a conserved protein module found in chromatin regulatory complexes
Proc.Natl.Acad.Sci.Usa, 103:2057-2062, 2006

PubMed Abstract: The SWIRM domain is a module found in the Swi3 and Rsc8 subunits of SWI/SNF-family chromatin remodeling complexes, and the Ada2 and BHC110/LSD1 subunits of chromatin modification complexes. Here we report the high-resolution crystal structure of the SWIRM domain from Swi3 and characterize the in vitro and in vivo function of the SWIRM domains from Saccharomyces cerevisiae Swi3 and Rsc8. The Swi3 SWIRM forms a four-helix bundle containing a pseudo 2-fold axis and a helix-turn-helix motif commonly found in DNA-binding proteins. We show that the Swi3 SWIRM binds free DNA and mononucleosomes with high and comparable affinity and that a subset of Swi3 substitution mutants that display growth defects in vivo also show impaired DNA-binding activity in vitro, consistent with a nucleosome targeting function of this domain. Genetic and biochemical studies also reveal that the Rsc8 and Swi3 SWIRM domains are essential for the proper assembly and in vivo functions of their respective complexes. Together, these studies identify the SWIRM domain as an essential multifunctional module for the regulation of gene expression.

PubMed: 16461455
DOI: 10.1073/pnas.0510949103

実験手法

X-RAY DIFFRACTION (1.4 Å)