2FPQ

Crystal Structure of Botulinum Neurotoxin Type D Light Chain

2FPQ の概要

• エントリーDOI: 10.2210/pdb2fpq/pdb
• 分子名称: BOTULINUM NEUROTOXIN D LIGHT CHAIN, ZINC ION, POTASSIUM ION, ... (4 entities in total)
• 機能のキーワード: hexxh metalloprotease, toxin
• 由来する生物種: Clostridium botulinum
• 細胞内の位置: Botulinum neurotoxin D light chain: Secreted. Botulinum neurotoxin D heavy chain: Secreted: P19321
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 50761.47

構造登録者

Arndt, J.W.,Chai, Q.,Christian, T.,Stevens, R.C. (登録日: 2006-01-16, 公開日: 2006-03-21, 最終更新日: 2024-02-14)

主引用文献

Arndt, J.W.,Chai, Q.,Christian, T.,Stevens, R.C.
Structure of Botulinum Neurotoxin Type D Light Chain at 1.65 A Resolution: Repercussions for VAMP-2 Substrate Specificity(,).
Biochemistry, 45:3255-3262, 2006

PubMed Abstract: The seven serotypes (A-G) of botulinum neurotoxins (BoNTs) function through their proteolytic cleavage of one of three proteins (SNAP-25, Syntaxin, and VAMP) that form the SNARE complex required for synaptic vesicle fusion. The different BoNTs have very specific protease recognition requirements, between 15 and 50 amino acids in length depending on the serotype. However, the structural details involved in substrate recognition remain largely unknown. Here is reported the 1.65 A resolution crystal structure of the catalytic domain of BoNT serotype D (BoNT/D-LC), providing insight into the protein-protein binding interaction and final proteolysis of VAMP-2. Structural analysis has identified a hydrophobic pocket potentially involved in substrate recognition of the P1' VAMP residue (Leu 60) and a second remote site for recognition of the V1 SNARE motif that is critical for activity. A structural comparison of BoNT/D-LC with BoNT/F-LC that also recognizes VAMP-2 one residue away from the BoNT/D-LC site provides additional molecular details about the unique serotype specific activities. In particular, BoNT/D prefers a hydrophobic interaction for the V1 motif of VAMP-2, while BoNT/F adopts a more hydrophilic strategy for recognition of the same V1 motif.

PubMed: 16519520
DOI: 10.1021/bi052518r

実験手法

X-RAY DIFFRACTION (1.65 Å)