2FN6

Helicobacter pylori PseC, aminotransferase involved in the biosynthesis of pseudoaminic acid

2FN6 の概要

• エントリーDOI: 10.2210/pdb2fn6/pdb
• 分子名称: AMINOTRANSFERASE, PHOSPHATE ION (3 entities in total)
• 機能のキーワード: aminotransferase, pseudoaminic acid biosynthesis, flagellar modification, structural genomics, montreal-kingston bacterial structural genomics initiative, bsgi, transferase
• 由来する生物種: Helicobacter pylori
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 85107.32

構造登録者

Cygler, M.,Lunin, V.V.,Matte, A.,Montreal-Kingston Bacterial Structural Genomics Initiative (BSGI) (登録日: 2006-01-10, 公開日: 2006-01-24, 最終更新日: 2023-08-30)

主引用文献

Schoenhofen, I.C.,Lunin, V.V.,Julien, J.P.,Li, Y.,Ajamian, E.,Matte, A.,Cygler, M.,Brisson, J.R.,Aubry, A.,Logan, S.M.,Bhatia, S.,Wakarchuk, W.W.,Young, N.M.
Structural and Functional Characterization of PseC, an Aminotransferase Involved in the Biosynthesis of Pseudaminic Acid, an Essential Flagellar Modification in Helicobacter pylori
J.Biol.Chem., 281:8907-8916, 2006

PubMed Abstract: Helicobacter pylori flagellin is heavily glycosylated with the novel sialic acid-like nonulosonate, pseudaminic acid (Pse). The glycosylation process is essential for assembly of functional flagellar filaments and consequent bacterial motility. Because motility is a key virulence factor for this and other important pathogens, the Pse biosynthetic pathway offers potential for novel therapeutic targets. From recent NMR analyses, we determined that the conversion of UDP-alpha-D-Glc-NAc to the central intermediate in the pathway, UDP-4-amino-4,6-dideoxy-beta-L-AltNAc, proceeds by formation of UDP-2-acetamido-2,6-dideoxy-beta-L-arabino-4-hexulose by the dehydratase/epimerase PseB (HP0840) followed with amino transfer by the aminotransferase, PseC (HP0366). The central role of PseC in the H. pylori Pse biosynthetic pathway prompted us to determine crystal structures of the native protein, its complexes with pyridoxal phosphate alone and in combination with the UDP-4-amino-4,6-dideoxy-beta-L-AltNAc product, the latter being converted to the external aldimine form in the active site of the enzyme. In the binding site, the AltNAc sugar ring adopts a 4C1 chair conformation, which is different from the predominant 1C4 form found in solution. The enzyme forms a homodimer where each monomer contributes to the active site, and these structures have permitted the identification of key residues involved in stabilization, and possibly catalysis, of the beta-L-arabino intermediate during the amino transfer reaction. The essential role of Lys183 in the catalytic event was confirmed by site-directed mutagenesis. This work presents for the first time a nucleotide-sugar aminotransferase co-crystallized with its natural ligand, and, in conjunction with the recent functional characterization of this enzyme, these results will assist in elucidating the aminotransferase reaction mechanism within the Pse biosynthetic pathway.

PubMed: 16421095
DOI: 10.1074/jbc.M512987200

実験手法

X-RAY DIFFRACTION (2.483 Å)