2FLH

Crystal structure of cytokinin-specific binding protein from mung bean in complex with cytokinin

2FLH の概要

• エントリーDOI: 10.2210/pdb2flh/pdb
• 関連するPDBエントリー: 1BV1 1ICX 1IFV 1XDF
• 分子名称: cytokinin-specific binding protein, (2E)-2-methyl-4-(9H-purin-6-ylamino)but-2-en-1-ol, SODIUM ION, ... (4 entities in total)
• 機能のキーワード: cytokinins, zeatin, pathogenesis-related proteins, multiple-ligand binding, plant protein
• 由来する生物種: Vigna radiata
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 72470.57

構造登録者

Pasternak, O.,Bujacz, G.D.,Sikorski, M.M.,Jaskolski, M. (登録日: 2006-01-06, 公開日: 2006-11-21, 最終更新日: 2024-02-14)

主引用文献

Pasternak, O.,Bujacz, G.D.,Fujimoto, Y.,Hashimoto, Y.,Jelen, F.,Otlewski, J.,Sikorski, M.M.,Jaskolski, M.
Crystal Structure of Vigna radiata Cytokinin-Specific Binding Protein in Complex with Zeatin.
Plant Cell, 18:2622-2634, 2006

PubMed Abstract: The cytosolic fraction of Vigna radiata contains a 17-kD protein that binds plant hormones from the cytokinin group, such as zeatin. Using recombinant protein and isothermal titration calorimetry as well as fluorescence measurements coupled with ligand displacement, we have reexamined the K(d) values and show them to range from approximately 10(-6) M (for 4PU30) to 10(-4) M (for zeatin) for 1:1 stoichiometry complexes. In addition, we have crystallized this cytokinin-specific binding protein (Vr CSBP) in complex with zeatin and refined the structure to 1.2 A resolution. Structurally, Vr CSBP is similar to plant pathogenesis-related class 10 (PR-10) proteins, despite low sequence identity (<20%). This unusual fold conservation reinforces the notion that classic PR-10 proteins have evolved to bind small-molecule ligands. The fold consists of an antiparallel beta-sheet wrapped around a C-terminal alpha-helix, with two short alpha-helices closing a cavity formed within the protein core. In each of the four independent CSBP molecules, there is a zeatin ligand located deep in the cavity with conserved conformation and protein-ligand interactions. In three cases, an additional zeatin molecule is found in variable orientation but with excellent definition in electron density, which plugs the entrance to the binding pocket, sealing the inner molecule from contact with bulk solvent.

PubMed: 16998071
DOI: 10.1105/tpc.105.037119

実験手法

X-RAY DIFFRACTION (1.2 Å)