2FJ7

Crystal structure of Nucleosome Core Particle Containing a Poly (dA.dT) Sequence Element

2FJ7 の概要

• エントリーDOI: 10.2210/pdb2fj7/pdb
• 関連するPDBエントリー: 1AOI 1KX5
• 分子名称: 147 bp DNA containing 16 bp poly dA element, 147 bp DNA containing 16 bp poly dT element, histone H3, ... (6 entities in total)
• 機能のキーワード: protein-dna complex, narrow minor groove, structural protein-dna complex, structural protein/dna
• 由来する生物種: Xenopus laevis (African clawed frog); 詳細
• 細胞内の位置: Nucleus: P62799 P02281
• タンパク質・核酸の鎖数: 10
• 化学式量合計: 199506.17

構造登録者

Bao, Y.,White, C.L.,Luger, K. (登録日: 2005-12-31, 公開日: 2006-09-26, 最終更新日: 2024-02-14)

主引用文献

Bao, Y.,White, C.L.,Luger, K.
Nucleosome Core Particles Containing a Poly(dA.dT) Sequence Element Exhibit a Locally Distorted DNA Structure.
J.Mol.Biol., 361:617-624, 2006

PubMed Abstract: Poly(dA.dT) DNA sequence elements are thought to promote transcription by either excluding nucleosomes or by altering their structural or dynamic properties. Here, the stability and structure of a defined nucleosome core particle containing a 16 base-pair poly(dA.dT) element (A16 NCP) was investigated. The A16 NCP requires a significantly higher temperature for histone octamer sliding in vitro compared to comparable nucleosomes that do not contain a poly(dA.dT) element. Fluorescence resonance energy transfer showed that the interactions between the nucleosomal DNA ends and the histone octamer were destabilized in A16 NCP. The crystal structure of A16 NCP was determined to a resolution of 3.2 A. The overall structure was maintained except for local deviations in DNA conformation. These results are consistent with previous in vivo and in vitro observations that poly(dA.dT) elements cause only modest changes in DNA accessibility and modest increases in steady-state transcription levels.

PubMed: 16860337
DOI: 10.1016/j.jmb.2006.06.051

実験手法

X-RAY DIFFRACTION (3.2 Å)