2FFL

Crystal Structure of Dicer from Giardia intestinalis

2FFL の概要

• エントリーDOI: 10.2210/pdb2ffl/pdb
• 分子名称: Dicer, MANGANESE (II) ION (3 entities in total)
• 機能のキーワード: rna interference; rnai; paz domain, translation, hydrolase
• 由来する生物種: Giardia intestinalis
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 332902.86

構造登録者

Doudna, J.A.,MacRae, I.J.,Adams, P.D. (登録日: 2005-12-19, 公開日: 2006-01-24, 最終更新日: 2024-02-14)

主引用文献

MacRae, I.J.,Zhou, K.,Li, F.,Repic, A.,Brooks, A.N.,Cande, W.Z.,Adams, P.D.,Doudna, J.A.
Structural Basis of Double-Stranded RNA Processing by Dicer
Science, 311:195-198, 2006

PubMed Abstract: The specialized ribonuclease Dicer initiates RNA interference by cleaving double-stranded RNA (dsRNA) substrates into small fragments about 25 nucleotides in length. In the crystal structure of an intact Dicer enzyme, the PAZ domain, a module that binds the end of dsRNA, is separated from the two catalytic ribonuclease III (RNase III) domains by a flat, positively charged surface. The 65 angstrom distance between the PAZ and RNase III domains matches the length spanned by 25 base pairs of RNA. Thus, Dicer itself is a molecular ruler that recognizes dsRNA and cleaves a specified distance from the helical end.

PubMed: 16410517
DOI: 10.1126/science.1121638

実験手法

X-RAY DIFFRACTION (3.33 Å)