2FF3

Crystal structure of Gelsolin domain 1:N-wasp V2 motif hybrid in complex with actin

2FF3 の概要

• エントリーDOI: 10.2210/pdb2ff3/pdb
• 分子名称: Gelsolin, Neural Wiskott-Aldrich syndrome protein, Actin, alpha skeletal muscle, ... (6 entities in total)
• 機能のキーワード: protein-protein complex, structural protein-contractile protein complex, structural protein/contractile protein
• 由来する生物種: Homo sapiens (human); 詳細
• 細胞内の位置: Isoform 2: Cytoplasm, cytoskeleton. Isoform 1: Secreted: P06396; Cytoplasm, cytoskeleton (By similarity): O00401; Cytoplasm, cytoskeleton: P68135
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 60813.50

構造登録者

Xue, B.,Aguda, A.H.,Robinson, R.C. (登録日: 2005-12-19, 公開日: 2006-03-21, 最終更新日: 2024-10-09)

主引用文献

Aguda, A.H.,Xue, B.,Irobi, E.,Preat, T.,Robinson, R.C.
The Structural Basis of Actin Interaction with Multiple WH2/beta-Thymosin Motif-Containing Proteins
Structure, 14:469-476, 2006

PubMed Abstract: Participation of actin in cellular processes relies on the dynamics of filament assembly. Filament elongation is fed by monomeric actin in complex with either profilin or a Wiscott-Aldrich syndrome protein (WASP) homology domain 2 (WH2)/beta-thymosin (betaT) domain. WH2/betaT motif repetition (typified by ciboulot) or combination with nonrelated domains (as found in N-WASP) results in proteins that yield their actin to filament elongation. Here, we report the crystal structures of actin bound hybrid proteins, constructed between gelsolin and WH2/betaT domains from ciboulot or N-WASP. We observe the C-terminal half of ciboulot domain 2 bound to actin. In solution, we show that cibolout domains 2 and 3 bind to both G- and F-actin, and that whole ciboulot forms a complex with two actin monomers. In contrast, the analogous portion of N-WASP WH2 domain 2 is detached from actin, indicating that the C-terminal halves of the betaT and WH2 motifs are not functionally analogous.

PubMed: 16531231
DOI: 10.1016/j.str.2005.12.011

実験手法

X-RAY DIFFRACTION (2 Å)