2FEY

The structure of stem loop IV of Tetrahymena telomerase RNA

2FEY の概要

• エントリーDOI: 10.2210/pdb2fey/pdb
• 分子名称: stem-loop IV of Tetrahymena telomerase RNA (1 entity in total)
• 機能のキーワード: telomerase rna, tetrahymena, stem-loop iv, heptaloop, rna
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 13685.10

構造登録者

Chen, Y.,Fender, J.,Legassie, J.D.,Jarstfer, M.B.,Bryan, T.M.,Varani, G. (登録日: 2005-12-16, 公開日: 2006-06-27, 最終更新日: 2024-05-29)

主引用文献

Chen, Y.,Fender, J.,Legassie, J.D.,Jarstfer, M.B.,Bryan, T.M.,Varani, G.
Structure of stem-loop IV of Tetrahymena telomerase RNA.
Embo J., 25:3156-3166, 2006

PubMed Abstract: Conserved domains within the RNA component of telomerase provide the template for reverse transcription, recruit protein components to the holoenzyme and are required for enzymatic activity. Among the functionally essential domains in ciliate telomerase RNA is stem-loop IV, which strongly stimulates telomerase activity and processivity even when provided in trans. The NMR structure of Tetrahymena thermophila stem-loop IV shows a highly structured distal stem-loop linked to a conformationally flexible template-proximal region by a bulge that severely kinks the entire RNA. Through extensive structure-function studies, we identify residues that contribute to both these structural features and to enzymatic activity, with no apparent effect on the binding of TERT protein. We propose that the bending induced by the GA bulge and the flexibility of the template-proximal region allow positioning of the prestructured apical loop during the catalytic cycle.

PubMed: 16778765
DOI: 10.1038/sj.emboj.7601195

実験手法

SOLUTION NMR