2FE6

P450CAM from Pseudomonas putida reconstituted with manganic protoporphyrin IX

2FE6 の概要

• エントリーDOI: 10.2210/pdb2fe6/pdb
• 分子名称: Cytochrome P450-cam, POTASSIUM ION, PROTOPORPHYRIN IX CONTAINING MN, ... (4 entities in total)
• 機能のキーワード: mono-oxygenase, heme, manganic, substrate-free, oxidoreductase
• 由来する生物種: Pseudomonas putida
• 細胞内の位置: Cytoplasm : P00183
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 48203.62

構造登録者

von Koenig, K.,Makris, T.M.,Sligar, S.G.,Schlichting, I. (登録日: 2005-12-15, 公開日: 2006-03-14, 最終更新日: 2023-08-30)

主引用文献

Makris, T.M.,Koenig, K.,Schlichting, I.,Sligar, S.G.
The status of high-valent metal oxo complexes in the P450 cytochromes.
J.Inorg.Biochem., 100:507-518, 2006

PubMed Abstract: The oxidative prowess of the P450 cytochromes in physiological reactions is attributed to the production of a high-valent iron-oxo complex, or Compound I intermediate, in the reaction cycle. Despite many years of study, however, the full electronic description of this fleeting intermediate still remains an active area of study. In this manuscript, the current status of the isolation and characterization of the P450 oxo-Fe(IV) is examined and compared to analogous states in related heme enzymes. In addition, the utilization of cofactor exchange to stabilize high-valent oxo-states in the P450 is addressed. Structural and spectroscopic studies on manganese reconstituted P450, and its corresponding oxo-complex, are presented.

PubMed: 16510191
DOI: 10.1016/j.jinorgbio.2006.01.025

実験手法

X-RAY DIFFRACTION (1.5 Å)