2FDP
Crystal structure of beta-secretase complexed with an amino-ethylene inhibitor
Summary for 2FDP
| Entry DOI | 10.2210/pdb2fdp/pdb |
| Descriptor | Beta-secretase 1, N1-((2S,3S,5R)-3-AMINO-6-(4-FLUOROPHENYLAMINO)-5-METHYL-6-OXO-1-PHENYLHEXAN-2-YL)-N3,N3-DIPROPYLISOPHTHALAMIDE (3 entities in total) |
| Functional Keywords | aspartyl protease, bace, hydrolase |
| Biological source | Homo sapiens (human) |
| Cellular location | Membrane; Single-pass type I membrane protein: P56817 |
| Total number of polymer chains | 3 |
| Total formula weight | 131449.37 |
| Authors | Yang, W.,Lu, W.,Lu, Y.,Zhong, M.,Sun, J.,Thomas, A.E.,Wilkinson, J.M.,Fucini, R.V.,Lam, M.,Randal, M.,Shi, X.P.,Jacobs, J.W.,McDowell, R.S.,Gordon, E.M.,Ballinger, M.D. (deposition date: 2005-12-14, release date: 2006-01-24, Last modification date: 2024-10-30) |
| Primary citation | Yang, W.,Lu, W.,Lu, Y.,Zhong, M.,Sun, J.,Thomas, A.E.,Wilkinson, J.M.,Fucini, R.V.,Lam, M.,Randal, M.,Shi, X.P.,Jacobs, J.W.,McDowell, R.S.,Gordon, E.M.,Ballinger, M.D. Aminoethylenes: a tetrahedral intermediate isostere yielding potent inhibitors of the aspartyl protease BACE-1. J.Med.Chem., 49:839-842, 2006 Cited by PubMed Abstract: A series of novel beta-site amyloid precursor protein cleaving enzyme (BACE-1) inhibitors containing an aminoethylene (AE) tetrahedral intermediate isostere were synthesized and evaluated in comparison to corresponding hydroxyethylene (HE) compounds. Enzymatic inhibitory values were similar for both isosteres, as were structure-activity relationships with respect to stereochemical preference and substituent variation (P2/P3, P1, and P2'); however, the AE compounds were markedly more potent in a cell-based assay for reduction of beta-secretase activity. The incorporation of preferred P2/P3, P1, and P2' substituents into the AE pharmacophore yielded compound 7, which possessed enzymatic and cell assay IC(50)s of 26 nM and 180 nM, respectively. A three-dimensional crystal structure of 7 in complex with BACE-1 revealed that the amino group of the inhibitor core engages the catalytic aspartates in a manner analogous to hydroxyl groups in HE inhibitors. The AE isostere class represents a promising advance in the development of BACE-1 inhibitors. PubMed: 16451048DOI: 10.1021/jm0509142 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (2.5 Å) |
Structure validation
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