2FBZ

Heme-No complex in a bacterial Nitric Oxide Synthase

2FBZ の概要

• エントリーDOI: 10.2210/pdb2fbz/pdb
• 関連するPDBエントリー: 1M7Z
• 分子名称: Nitric oxide synthase oxygenase, PROTOPORPHYRIN IX CONTAINING FE, 2-AMINO-6-(1,2-DIHYDROXY-PROPYL)-7,8-DIHYDRO-6H-PTERIDIN-4-ONE, ... (6 entities in total)
• 機能のキーワード: heme-no complex, nitric oxide synthase, oxidoreductase
• 由来する生物種: Bacillus subtilis
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 43173.15

構造登録者

Pant, K.,Crane, B.R. (登録日: 2005-12-10, 公開日: 2006-08-29, 最終更新日: 2024-02-14)

主引用文献

Pant, K.,Crane, B.R.
Nitrosyl-heme structures of Bacillus subtilis nitric oxide synthase have implications for understanding substrate oxidation.
Biochemistry, 45:2537-2544, 2006

PubMed Abstract: The crystal structures of nitrosyl-heme complexes of a prokaryotic nitric oxide synthase (NOS) from Bacillus subtilis (bsNOS) reveal changes in active-site hydrogen bonding in the presence of the intermediate N(omega)-hydroxy-l-arginine (NOHA) compared to the substrate l-arginine (l-Arg). Correlating with a Val-to-Ile residue substitution in the bsNOS heme pocket, the Fe(II)-NO complex with both l-Arg and NOHA is more bent than the Fe(II)-NO, l-Arg complex of mammalian eNOS [Li, H., Raman, C. S., Martasek, P., Masters, B. S. S., and Poulos, T. L. (2001) Biochemistry 40, 5399-5406]. Structures of the Fe(III)-NO complex with NOHA show a nearly linear nitrosyl group, and in one subunit, partial nitrosation of bound NOHA. In the Fe(II)-NO complexes, the protonated NOHA N(omega) atom forms a short hydrogen bond with the heme-coordinated NO nitrogen, but active-site water molecules are out of hydrogen bonding range with the distal NO oxygen. In contrast, the l-Arg guanidinium interacts more weakly and equally with both NO atoms, and an active-site water molecule hydrogen bonds to the distal NO oxygen. This difference in hydrogen bonding to the nitrosyl group by the two substrates indicates that interactions provided by NOHA may preferentially stabilize an electrophilic peroxo-heme intermediate in the second step of NOS catalysis.

PubMed: 16489746
DOI: 10.1021/bi0518848

実験手法

X-RAY DIFFRACTION (2.1 Å)