2FBD
The crystallographic structure of the digestive lysozyme 1 from Musca domestica at 1.90 Ang.
Summary for 2FBD
| Entry DOI | 10.2210/pdb2fbd/pdb |
| Related | 1HEW |
| Descriptor | Lysozyme 1, SULFATE ION, DI(HYDROXYETHYL)ETHER, ... (4 entities in total) |
| Functional Keywords | digestive lysozime, musca domestica, hydrolase |
| Biological source | Musca domestica (house fly) |
| Total number of polymer chains | 2 |
| Total formula weight | 28157.08 |
| Authors | Cancado, F.C.,Marana, S.R.,Barbosa, J.A.R.G. (deposition date: 2005-12-09, release date: 2006-12-12, Last modification date: 2024-10-30) |
| Primary citation | Marana, S.R.,Cancado, F.C.,Valero, A.A.,Ferreira, C.,Terra, W.R.,Barbosa, J.A.R.G. Crystallization, data collection and phasing of two digestive lysozymes from Musca domestica. Acta Crystallogr.,Sect.F, 62:750-752, 2006 Cited by PubMed Abstract: Lysozymes are mostly known for their defensive role against bacteria, but in several animals lysozymes have a digestive function. Here, the initial crystallographic characterization of two digestive lysozymes from Musca domestica are presented. The proteins were crystallized using the sitting-drop vapour-diffusion method in the presence of ammonium sulfate or PEG/2-propanol as the precipitant. X-ray diffraction data were collected to a maximum resolution of 1.9 angstroms using synchrotron radiation. The lysozyme 1 and 2 crystals belong to the monoclinic space group P2(1) (unit-cell parameters a = 36.52, b = 79.44, c = 45.20 angstroms, beta = 102.97 degrees) and the orthorhombic space group P2(1)2(1)2 (unit-cell parameters a = 73.90, b = 96.40, c = 33.27 angstroms), respectively. The crystal structures were solved by molecular replacement and structure refinement is in progress. PubMed: 16880547DOI: 10.1107/S1744309106024201 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (1.9 Å) |
Structure validation
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