2FBD

The crystallographic structure of the digestive lysozyme 1 from Musca domestica at 1.90 Ang.

2FBD の概要

• エントリーDOI: 10.2210/pdb2fbd/pdb
• 関連するPDBエントリー: 1HEW
• 分子名称: Lysozyme 1, SULFATE ION, DI(HYDROXYETHYL)ETHER, ... (4 entities in total)
• 機能のキーワード: digestive lysozime, musca domestica, hydrolase
• 由来する生物種: Musca domestica (house fly)
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 28157.08

構造登録者

Cancado, F.C.,Marana, S.R.,Barbosa, J.A.R.G. (登録日: 2005-12-09, 公開日: 2006-12-12, 最終更新日: 2024-10-30)

主引用文献

Marana, S.R.,Cancado, F.C.,Valero, A.A.,Ferreira, C.,Terra, W.R.,Barbosa, J.A.R.G.
Crystallization, data collection and phasing of two digestive lysozymes from Musca domestica.
Acta Crystallogr.,Sect.F, 62:750-752, 2006

PubMed Abstract: Lysozymes are mostly known for their defensive role against bacteria, but in several animals lysozymes have a digestive function. Here, the initial crystallographic characterization of two digestive lysozymes from Musca domestica are presented. The proteins were crystallized using the sitting-drop vapour-diffusion method in the presence of ammonium sulfate or PEG/2-propanol as the precipitant. X-ray diffraction data were collected to a maximum resolution of 1.9 angstroms using synchrotron radiation. The lysozyme 1 and 2 crystals belong to the monoclinic space group P2(1) (unit-cell parameters a = 36.52, b = 79.44, c = 45.20 angstroms, beta = 102.97 degrees) and the orthorhombic space group P2(1)2(1)2 (unit-cell parameters a = 73.90, b = 96.40, c = 33.27 angstroms), respectively. The crystal structures were solved by molecular replacement and structure refinement is in progress.

PubMed: 16880547
DOI: 10.1107/S1744309106024201

実験手法

X-RAY DIFFRACTION (1.9 Å)