2F66

Structure of the ESCRT-I endosomal trafficking complex

2F66 の概要

• エントリーDOI: 10.2210/pdb2f66/pdb
• 関連するPDBエントリー: 1U5T 1UZX 1W7P 1XB4
• 分子名称: Suppressor protein STP22 of temperature-sensitive alpha-factor receptor and arginine permease, Vacuolar protein sorting-associated protein VPS28, Protein SRN2, ... (5 entities in total)
• 機能のキーワード: endosome, trafficking complex, vps23, vps28, vps37, vacuolar protein sorting, escrt protein complexes, endosomal sorting complex required for transport, escrt-i, ubiquitin, tsg101, transport protein
• 由来する生物種: Saccharomyces cerevisiae (baker's yeast); 詳細
• 細胞内の位置: Cytoplasm: P25604 Q02767 Q99176
• タンパク質・核酸の鎖数: 6
• 化学式量合計: 61554.80

構造登録者

Kostelansky, M.S.,Lee, S.,Kim, J.,Hurley, J.H. (登録日: 2005-11-28, 公開日: 2006-04-18, 最終更新日: 2023-08-23)

主引用文献

Kostelansky, M.S.,Sun, J.,Lee, S.,Kim, J.,Ghirlando, R.,Hierro, A.,Emr, S.D.,Hurley, J.H.
Structural and functional organization of the ESCRT-I trafficking complex.
Cell(Cambridge,Mass.), 125:113-126, 2006

PubMed Abstract: The endosomal sorting complex required for transport (ESCRT) complexes are central to receptor downregulation, lysosome biogenesis, and budding of HIV. The yeast ESCRT-I complex contains the Vps23, Vps28, and Vps37 proteins, and its assembly is directed by the C-terminal steadiness box of Vps23, the N-terminal half of Vps28, and the C-terminal half of Vps37. The crystal structures of a Vps23:Vps28 core subcomplex and the Vps23:Vps28:Vps37 core were solved at 2.1 and 2.8 A resolution. Each subunit contains a structurally similar pair of helices that form the core. The N-terminal domain of Vps28 has a hydrophobic binding site on its surface that is conformationally dynamic. The C-terminal domain of Vps28 binds the ESCRT-II complex. The structure shows how ESCRT-I is assembled by a compact core from which the Vps23 UEV domain, the Vps28 C domain, and other domains project to bind their partners.

PubMed: 16615894
DOI: 10.1016/j.cell.2006.01.049

実験手法

X-RAY DIFFRACTION (2.8 Å)