2F5W

Cross-linked barnase soaked in 3 M thiourea

Summary for 2F5W

Entry DOI	10.2210/pdb2f5w/pdb
Related	2F4Y 2F56 2F5M
Descriptor	Ribonuclease, ZINC ION, THIOUREA, ... (4 entities in total)
Functional Keywords	denaturation, lysozyme, barnase, cross-linked crystals, glutaraldehyde, urea, thiourea, bromoethanol, hydrolase
Biological source	Bacillus amyloliquefaciens
Cellular location	Secreted: P00648
Total number of polymer chains	3
Total formula weight	36740.07
Authors	Prange, T.,Salem, M. (deposition date: 2005-11-28, release date: 2006-04-25, Last modification date: 2023-08-23)
Primary citation	Salem, M.,Mauguen, Y.,Prange, T. On the edge of the denaturation process: Application of X-ray diffraction to barnase and lysozyme cross-linked crystals with denaturants in molar concentrations. Biochim.Biophys.Acta, 1764:903-912, 2006 Cited by PubMed: 16600702 DOI: 10.1016/j.bbapap.2006.02.009 PDB entries with the same primary citation
Experimental method	X-RAY DIFFRACTION (2.001 Å)

Structure validation