2F3Z

Calmodulin/IQ-AA domain complex

2F3Z の概要

• エントリーDOI: 10.2210/pdb2f3z/pdb
• 関連するPDBエントリー: 1CDL 1CDM 1PRW 2F3Y
• 分子名称: Calmodulin, Voltage-dependent L-type calcium channel alpha-1C subunit, CALCIUM ION, ... (4 entities in total)
• 機能のキーワード: calmodulin, calmodulin complex, calcium channnel, cav1.2, iq domain, iq-aa mutant domain, metal binding protein
• 由来する生物種: Homo sapiens (human); 詳細
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 19588.86

構造登録者

Fallon, J.L.,Quiocho, F.A. (登録日: 2005-11-22, 公開日: 2005-12-27, 最終更新日: 2023-08-23)

主引用文献

Fallon, J.L.,Halling, D.B.,Hamilton, S.L.,Quiocho, F.A.
Structure of Calmodulin Bound to the Hydrophobic IQ Domain of the Cardiac Ca(v)1.2 Calcium Channel.
Structure, 13:1881-1886, 2005

PubMed Abstract: Ca2+-dependent inactivation (CDI) and facilitation (CDF) of the Ca(v)1.2 Ca2+ channel require calmodulin binding to a putative IQ motif in the carboxy-terminal tail of the pore-forming subunit. We present the 1.45 A crystal structure of Ca2+-calmodulin bound to a 21 residue peptide corresponding to the IQ domain of Ca(v)1.2. This structure shows that parallel binding of calmodulin to the IQ domain is governed by hydrophobic interactions. Mutations of residues I1672 and Q1673 in the peptide to alanines, which abolish CDI but not CDF in the channel, do not greatly alter the structure. Both lobes of Ca2+-saturated CaM bind to the IQ peptide but isoleucine 1672, thought to form an intramolecular interaction that drives CDI, is buried. These findings suggest that this structure could represent the conformation that calmodulin assumes in CDF.

PubMed: 16338416
DOI: 10.1016/j.str.2005.09.021

実験手法

X-RAY DIFFRACTION (1.6 Å)