2EY4

Crystal Structure of a Cbf5-Nop10-Gar1 Complex

2EY4 の概要

• エントリーDOI: 10.2210/pdb2ey4/pdb
• 分子名称: Probable tRNA pseudouridine synthase B, small nucleolar rnp similar to gar1, Ribosome biogenesis protein Nop10, ... (5 entities in total)
• 機能のキーワード: trimeric complex, structural genomics, psi, protein structure initiative, southeast collaboratory for structural genomics, secsg, isomerase-biosynthetic protein complex, isomerase/biosynthetic protein
• 由来する生物種: Pyrococcus furiosus; 詳細
• タンパク質・核酸の鎖数: 6
• 化学式量合計: 107751.08

構造登録者

Rashid, R.,Liang, B.,Li, H.,Southeast Collaboratory for Structural Genomics (SECSG) (登録日: 2005-11-09, 公開日: 2006-01-24, 最終更新日: 2024-02-14)

主引用文献

Rashid, R.,Liang, B.,Baker, D.L.,Youssef, O.A.,He, Y.,Phipps, K.,Terns, R.M.,Terns, M.P.,Li, H.
Crystal structure of a Cbf5-Nop10-Gar1 complex and implications in RNA-guided pseudouridylation and dyskeratosis congenita.
Mol.Cell, 21:249-260, 2006

PubMed Abstract: H/ACA RNA-protein complexes, comprised of four proteins and an H/ACA guide RNA, modify ribosomal and small nuclear RNAs. The H/ACA proteins are also essential components of telomerase in mammals. Cbf5 is the H/ACA protein that catalyzes isomerization of uridine to pseudouridine in target RNAs. Mutations in human Cbf5 (dyskerin) lead to dyskeratosis congenita. Here, we describe the 2.1 A crystal structure of a specific complex of three archaeal H/ACA proteins, Cbf5, Nop10, and Gar1. Cbf5 displays structural properties that are unique among known pseudouridine synthases and are consistent with its distinct function in RNA-guided pseudouridylation. We also describe the previously unknown structures of both Nop10 and Gar1 and the structural basis for their essential roles in pseudouridylation. By using information from related structures, we have modeled the entire ribonucleoprotein complex including both guide and substrate RNAs. We have also identified a dyskeratosis congenita mutation cluster site within a modeled dyskerin structure.

PubMed: 16427014
DOI: 10.1016/j.molcel.2005.11.017

実験手法

X-RAY DIFFRACTION (2.11 Å)