2EX5

Group I Intron-encoded Homing Endonuclease I-CeuI Complexed With DNA

Summary for 2EX5

• Entry DOI: 10.2210/pdb2ex5/pdb
• Related: 1AF5 1BP7 1G9Y 1G9Z 1M5X 1MOW
• Descriptor: I-CeuI DNA target site, I-CeuI DNA target site, complementary strand, DNA endonuclease I-CeuI, ... (5 entities in total)
• Functional Keywords: homing endonuclease, laglidadg, homodimer, protein-dna complex, hydrolase-dna complex, hydrolase/dna
• Biological source: Chlamydomonas eugametos; More
• Total number of polymer chains: 4
• Total formula weight: 63378.90

Authors

Spiegel, P.C.,Stoddard, B.L. (deposition date: 2005-11-07, release date: 2006-05-23, Last modification date: 2024-04-03)

Primary citation

Spiegel, P.C.,Chevalier, B.,Sussman, D.,Turmel, M.,Lemieux, C.,Stoddard, B.L.
The structure of I-CeuI homing endonuclease: Evolving asymmetric DNA recognition from a symmetric protein scaffold.
Structure, 14:869-880, 2006

PubMed Abstract: Homing endonucleases are highly specific catalysts of DNA strand breaks, leading to the transfer of mobile intervening sequences containing the endonuclease ORF. We have determined the structure and DNA recognition behavior of I-CeuI, a homodimeric LAGLIDADG endonuclease from Chlamydomonas eugametos. This symmetric endonuclease displays unique structural elaborations on its core enzyme fold, and it preferentially cleaves a highly asymmetric target site. This latter property represents an early step, prior to gene fusion, in the generation of asymmetric DNA binding platforms from homodimeric ancestors. The divergence of the sequence, structure, and target recognition behavior of homing endonucleases, as illustrated by this study, leads to the invasion of novel genomic sites by mobile introns during evolution.

PubMed: 16698548
DOI: 10.1016/j.str.2006.03.009

Experimental method

X-RAY DIFFRACTION (2.2 Å)