2EX5

Group I Intron-encoded Homing Endonuclease I-CeuI Complexed With DNA

2EX5 の概要

• エントリーDOI: 10.2210/pdb2ex5/pdb
• 関連するPDBエントリー: 1AF5 1BP7 1G9Y 1G9Z 1M5X 1MOW
• 分子名称: I-CeuI DNA target site, I-CeuI DNA target site, complementary strand, DNA endonuclease I-CeuI, ... (5 entities in total)
• 機能のキーワード: homing endonuclease, laglidadg, homodimer, protein-dna complex, hydrolase-dna complex, hydrolase/dna
• 由来する生物種: Chlamydomonas eugametos; 詳細
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 63378.90

構造登録者

Spiegel, P.C.,Stoddard, B.L. (登録日: 2005-11-07, 公開日: 2006-05-23, 最終更新日: 2024-04-03)

主引用文献

Spiegel, P.C.,Chevalier, B.,Sussman, D.,Turmel, M.,Lemieux, C.,Stoddard, B.L.
The structure of I-CeuI homing endonuclease: Evolving asymmetric DNA recognition from a symmetric protein scaffold.
Structure, 14:869-880, 2006

PubMed Abstract: Homing endonucleases are highly specific catalysts of DNA strand breaks, leading to the transfer of mobile intervening sequences containing the endonuclease ORF. We have determined the structure and DNA recognition behavior of I-CeuI, a homodimeric LAGLIDADG endonuclease from Chlamydomonas eugametos. This symmetric endonuclease displays unique structural elaborations on its core enzyme fold, and it preferentially cleaves a highly asymmetric target site. This latter property represents an early step, prior to gene fusion, in the generation of asymmetric DNA binding platforms from homodimeric ancestors. The divergence of the sequence, structure, and target recognition behavior of homing endonucleases, as illustrated by this study, leads to the invasion of novel genomic sites by mobile introns during evolution.

PubMed: 16698548
DOI: 10.1016/j.str.2006.03.009

実験手法

X-RAY DIFFRACTION (2.2 Å)