2ERM

Solution structure of a biologically active human FGF-1 monomer, complexed to a hexasaccharide heparin-analogue

2ERM の概要

• エントリーDOI: 10.2210/pdb2erm/pdb
• 分子名称: Heparin-binding growth factor 1, 2-deoxy-2-(sulfoamino)-alpha-D-glucopyranose-(1-4)-2-O-sulfo-alpha-L-idopyranuronic acid-(1-4)-2-acetamido-2-deoxy-6-O-sulfo-alpha-D-glucopyranose-(1-4)-alpha-L-idopyranuronic acid-(1-4)-2-deoxy-2-(sulfoamino)-alpha-D-glucopyranose-(1-4)-2-O-sulfo-alpha-L-idopyranuronic acid, ISOPROPYL ALCOHOL (3 entities in total)
• 機能のキーワード: heparin-like hexasaccharide, fibroblast growth factor, protein-carbohydrate complex, hormone-growth factor complex, hormone/growth factor
• 由来する生物種: Homo sapiens (human)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 17242.99

構造登録者

Canales, A.,Lozano, R.,Nieto, P.M.,Martin-Lomas, M.,Gimenez-Gallego, G.,Jimenez-Barbero, J. (登録日: 2005-10-25, 公開日: 2006-10-03, 最終更新日: 2024-05-01)

主引用文献

Canales, A.,Lozano, R.,Lopez-Mendez, B.,Angulo, J.,Ojeda, R.,Nieto, P.M.,Martin-Lomas, M.,Gimenez-Gallego, G.,Jimenez-Barbero, J.
Solution NMR structure of a human FGF-1 monomer, activated by a hexasaccharide heparin-analogue.
Febs J., 273:4716-4727, 2006

PubMed Abstract: The 3D structure of a complex formed by the acidic fibroblast growth factor (FGF-1) and a specifically designed synthetic heparin hexasaccharide has been determined by NMR spectroscopy. This hexasaccharide can substitute natural heparins in FGF-1 mitogenesis assays, in spite of not inducing any apparent dimerization of the growth factor. The use of this well defined synthetic heparin analogue has allowed us to perform a detailed NMR structural analysis of the heparin-FGF interaction, overcoming the limitations of NMR to deal with the high molecular mass and heterogeneity of the FGF-1 oligomers formed in the presence of natural heparin fragments. Our results confirm that glycosaminoglycans induced FGF-1 dimerization either in a cis or trans disposition with respect to the heparin chain is not an absolute requirement for biological activity.

PubMed: 16995857
DOI: 10.1111/j.1742-4658.2006.05474.x

実験手法

SOLUTION NMR