2ERL

PHEROMONE ER-1 FROM

「1ERL」から置き換えられました

2ERL の概要

• エントリーDOI: 10.2210/pdb2erl/pdb
• 分子名称: MATING PHEROMONE ER-1, ETHANOL (3 entities in total)
• 機能のキーワード: pheromone
• 由来する生物種: Euplotes raikovi
• 細胞内の位置: Isoform 1: Secreted. Isoform 2: Cell membrane: P10774
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 4463.95

構造登録者

Anderson, D.H.,Weiss, M.S.,Eisenberg, D. (登録日: 1995-12-20, 公開日: 1996-07-11, 最終更新日: 2024-10-30)

主引用文献

Anderson, D.H.,Weiss, M.S.,Eisenberg, D.
A challenging case for protein crystal structure determination: the mating pheromone Er-1 from Euplotes raikovi.
Acta Crystallogr.,Sect.D, 52:469-480, 1996

PubMed Abstract: Four different phasing methods have been applied to the determination of the crystal structure of the 40 amino-acid mating pheromone of the unicellular ciliated protozoan Euplotes raikovi. The difficulties, failures and successes in attempts to solve the structure by: (1) molecular replacement, (2) direct phasing using the 'Shake and Bake' algorithm, (3) isomorphous replacement, and (4) multiple-wavelength anomalous dispersion are described. The structure was first solved by molecular replacement, and then was the first successful structure determination by 'Shake and Bake' without the direct involvement of its authors. A description of the current status of the high-resolution refinement of the structure is also given. The model is refined against 1 A resolution data to an R factor of 12.9%, and includes H atoms and discretely disordered side chains.

PubMed: 15299668
DOI: 10.1107/S0907444995014235

実験手法

X-RAY DIFFRACTION (1 Å)