2ERH

Crystal Structure of the E7_G/Im7_G complex; a designed interface between the colicin E7 DNAse and the Im7 immunity protein

2ERH の概要

• エントリーDOI: 10.2210/pdb2erh/pdb
• 関連するPDBエントリー: 7CEI
• 分子名称: Colicin E7 immunity protein, Colicin E7 (3 entities in total)
• 機能のキーワード: computational design, redesigned protein-protein interface, hydrogen bond network, specificity, molecular recognition, protein complex, hydrolase-hydrolase inhibitor complex, hydrolase/hydrolase inhibitor
• 由来する生物種: Escherichia coli; 詳細
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 24626.58

構造登録者

Joachimiak, L.A.,Kortemme, T.,Stoddard, B.L.,Baker, D. (登録日: 2005-10-24, 公開日: 2006-07-25, 最終更新日: 2023-08-23)

主引用文献

Joachimiak, L.A.,Kortemme, T.,Stoddard, B.L.,Baker, D.
Computational Design of a New Hydrogen Bond Network and at Least a 300-fold Specificity Switch at a Protein-Protein Interface.
J.Mol.Biol., 361:195-208, 2006

PubMed Abstract: The redesign of protein-protein interactions is a stringent test of our understanding of molecular recognition and specificity. Previously we engineered a modest specificity switch into the colicin E7 DNase-Im7 immunity protein complex by identifying mutations that are disruptive in the native complex, but can be compensated by mutations on the interacting partner. Here we extend the approach by systematically sampling alternate rigid body orientations to optimize the interactions in a binding mode specific manner. Using this protocol we designed a de novo hydrogen bond network at the DNase-immunity protein interface and confirmed the design with X-ray crystallographic analysis. Subsequent design of the second shell of interactions guided by insights from the crystal structure on tightly bound water molecules, conformational strain, and packing defects yielded new binding partners that exhibited specificities of at least 300-fold between the cognate and the non-cognate complexes. This multi-step approach should be applicable to the design of polar protein-protein interactions and contribute to the re-engineering of regulatory networks mediated by protein-protein interactions.

PubMed: 16831445
DOI: 10.1016/j.jmb.2006.05.022

実験手法

X-RAY DIFFRACTION (2 Å)