2EHB

The structure of the C-terminal domain of the protein kinase AtSOS2 bound to the calcium sensor AtSOS3

2EHB の概要

• エントリーDOI: 10.2210/pdb2ehb/pdb
• 分子名称: Calcineurin B-like protein 4, CBL-interacting serine/threonine-protein kinase 24, CALCIUM ION, ... (4 entities in total)
• 機能のキーワード: protein complex, ca(ii) ions bound to sos3 (ef-hands 1 and 4), sos2 fisl motif, sos2 phosphatase binding domain (ppi), signalling protein-transferase complex, signalling protein/transferase
• 由来する生物種: Arabidopsis thaliana (thale cress); 詳細
• 細胞内の位置: Cell membrane; Lipid-anchor: O81223
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 40494.89

構造登録者

Sanchez-Barrena, M.J. (登録日: 2007-03-06, 公開日: 2007-09-25, 最終更新日: 2024-04-03)

主引用文献

Sanchez-Barrena, M.J.,Fujii, H.,Angulo, I.,Martinez-Ripoll, M.,Zhu, J.K.,Albert, A.
The structure of the C-terminal domain of the protein kinase AtSOS2 bound to the calcium sensor AtSOS3
Mol.Cell, 26:427-435, 2007

PubMed Abstract: The plant SOS2 family of protein kinases and their interacting activators, the SOS3 family of calcium-binding proteins, function together in decoding calcium signals elicited by different environmental stimuli. SOS2 is activated by Ca-SOS3 and subsequently phosphorylates the ion transporter SOS1 to bring about cellular ion homeostasis under salt stress. In addition to possessing the kinase activity, members of the SOS2 family of protein kinases can bind to protein phosphatase 2Cs. The crystal structure of the binary complex of Ca-SOS3 with the C-terminal regulatory moiety of SOS2 resolves central questions regarding the dual function of SOS2 as a kinase and a phosphatase-binding protein. A comparison with the structure of unbound SOS3 reveals the basis of the molecular function of this family of kinases and their interacting calcium sensors. Furthermore, our study suggests that the structure of the phosphatase-interaction domain of SOS2 defines a scaffold module conserved from yeast to human.

PubMed: 17499048
DOI: 10.1016/j.molcel.2007.04.013

実験手法

X-RAY DIFFRACTION (2.1 Å)