2EB8

Crystal Structure of Cu(II)(Sal-Phe)/apo-Myoglobin

Summary for 2EB8

• Entry DOI: 10.2210/pdb2eb8/pdb
• Related: 2EB9
• Descriptor: Myoglobin, PHOSPHATE ION, (N-SALICYLIDEN-L-PHENYLALANATO)-COPPER(II), ... (4 entities in total)
• Functional Keywords: oxygen storage/transport, oxygen storage-transport complex
• Biological source: Physeter catodon (sperm whale)
• Total number of polymer chains: 1
• Total formula weight: 18171.83

Authors

Abe, S.,Okazaki, S.,Ueno, T.,Hikage, T.,Suzuki, A.,Yamane, T.,Watanabe, Y. (deposition date: 2007-02-07, release date: 2007-07-03, Last modification date: 2023-10-25)

Primary citation

Abe, S.,Ueno, T.,Reddy, P.A.N.,Okazaki, S.,Hikage, T.,Suzuki, A.,Yamane, T.,Nakajima, H.,Watanabe, Y.
Design and Structure Analysis of Artificial Metalloproteins: Selective Coordination of His64 to Copper Complexes with Square-Planar Structure in the apo-Myoglobin Scaffold
Inorg.Chem., 46:5137-5139, 2007

PubMed Abstract: apo-Myoglobin (apo-Mb) was reconstituted with three copper complexes: CuII(Sal-Phe) (1; Sal-Phe = N-salicylidene-L-phenylalanato), CuII(Sal-Leu) (2; Sal-Leu = N-salicylidene-L-leucinato), and CuII(Sal-Ala) (3; Sal-Ala = N-salicylidene-L-alanato). The crystal structures of 1.apo-Mb (1.65 Angstrom resolution) and 2.apo-Mb (1.8 Angstrom resolution) show that the coordination geometry around the CuII atom in apo-Mb is distorted square-planar with tridentate Sal-X and a Nepsilon atom of His64 in the apo-Mb cavity and the plane of these copper complexes is perpendicular to that of heme. These results suggest that the apo-Mb cavity can hold metal complexes with various coordination geometries.

PubMed: 17523632
DOI: 10.1021/ic070289m

Experimental method

X-RAY DIFFRACTION (1.65 Å)