2EB7

Crystal structure of the hypothetical regulator from Sulfolobus tokodaii 7

Summary for 2EB7

• Entry DOI: 10.2210/pdb2eb7/pdb
• Descriptor: 146aa long hypothetical transcriptional regulator (2 entities in total)
• Functional Keywords: transcription regulator, st1710, hypothetical regulator, mar family, structural genomics, nppsfa, national project on protein structural and functional analyses, riken structural genomics/proteomics initiative, rsgi, transcription
• Biological source: Sulfolobus tokodaii
• Total number of polymer chains: 1
• Total formula weight: 16879.56

Authors

Kumarevel, T.S.,Nishio, M.,Shinkai, A.,Yokoyama, S.,RIKEN Structural Genomics/Proteomics Initiative (RSGI) (deposition date: 2007-02-07, release date: 2007-11-06, Last modification date: 2024-03-13)

Primary citation

Kumarevel, T.,Tanaka, T.,Nishio, M.,Gopinath, S.C.B.,Takio, K.,Shinkai, A.,Kumar, P.K.R.,Yokoyama, S.
Crystal structure of the MarR family regulatory protein, ST1710, from Sulfolobus tokodaii strain 7
J.Struct.Biol., 161:9-17, 2008

PubMed Abstract: The emergence of bacterial resistance to multiple drugs poses a serious and growing health concern. Understanding and deciphering the mechanisms of these multiple drug resistance regulatory proteins through structural or biochemical means is an important endeavor. Here, we present the crystal structure of ST1710 from Sulfolobus tokodaii strain 7 in two different crystal forms, at 1.80 and 2.0A, respectively. The overall structure of the ST1710 dimer shares the topology of the MarR family of proteins, with each subunit containing a winged helix-turn-helix DNA-binding motif. We also show the protein-DNA interactions by biochemical methods. Our molecular modeling analysis suggested that Asp88 and Arg90 are the key residues in ST1710 involved in the protein-DNA interactions.

PubMed: 17933554
DOI: 10.1016/j.jsb.2007.08.017

Experimental method

X-RAY DIFFRACTION (1.8 Å)