2EB7

Crystal structure of the hypothetical regulator from Sulfolobus tokodaii 7

2EB7 の概要

• エントリーDOI: 10.2210/pdb2eb7/pdb
• 分子名称: 146aa long hypothetical transcriptional regulator (2 entities in total)
• 機能のキーワード: transcription regulator, st1710, hypothetical regulator, mar family, structural genomics, nppsfa, national project on protein structural and functional analyses, riken structural genomics/proteomics initiative, rsgi, transcription
• 由来する生物種: Sulfolobus tokodaii
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 16879.56

構造登録者

Kumarevel, T.S.,Nishio, M.,Shinkai, A.,Yokoyama, S.,RIKEN Structural Genomics/Proteomics Initiative (RSGI) (登録日: 2007-02-07, 公開日: 2007-11-06, 最終更新日: 2024-03-13)

主引用文献

Kumarevel, T.,Tanaka, T.,Nishio, M.,Gopinath, S.C.B.,Takio, K.,Shinkai, A.,Kumar, P.K.R.,Yokoyama, S.
Crystal structure of the MarR family regulatory protein, ST1710, from Sulfolobus tokodaii strain 7
J.Struct.Biol., 161:9-17, 2008

PubMed Abstract: The emergence of bacterial resistance to multiple drugs poses a serious and growing health concern. Understanding and deciphering the mechanisms of these multiple drug resistance regulatory proteins through structural or biochemical means is an important endeavor. Here, we present the crystal structure of ST1710 from Sulfolobus tokodaii strain 7 in two different crystal forms, at 1.80 and 2.0A, respectively. The overall structure of the ST1710 dimer shares the topology of the MarR family of proteins, with each subunit containing a winged helix-turn-helix DNA-binding motif. We also show the protein-DNA interactions by biochemical methods. Our molecular modeling analysis suggested that Asp88 and Arg90 are the key residues in ST1710 involved in the protein-DNA interactions.

PubMed: 17933554
DOI: 10.1016/j.jsb.2007.08.017

実験手法

X-RAY DIFFRACTION (1.8 Å)