2E86

Azide bound to copper containing nitrite reductase from A. faecalis S-6

2E86 の概要

• エントリーDOI: 10.2210/pdb2e86/pdb
• 関連するPDBエントリー: 1SJM 1SNR
• 分子名称: Copper-containing nitrite reductase, COPPER (I) ION, COPPER (II) ION, ... (7 entities in total)
• 機能のキーワード: nitrite reductase, azide, inhibitor, kinetic studies, oxidoreductase
• 由来する生物種: Alcaligenes faecalis
• 細胞内の位置: Periplasm: P38501
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 110486.32

構造登録者

Tocheva, E.I.,Murphy, M.E.P. (登録日: 2007-01-19, 公開日: 2008-01-22, 最終更新日: 2024-03-13)

主引用文献

Tocheva, E.I.,Eltis, L.D.,Murphy, M.E.P.
Conserved Active Site Residues Limit Inhibition of a Copper-Containing Nitrite Reductase by Small Molecules.
Biochemistry, 47:4452-4460, 2008

PubMed Abstract: The interaction of copper-containing dissimilatory nitrite reductase from Alcaligenes faecalis S-6 ( AfNiR) with each of five small molecules was studied using crystallography and steady-state kinetics. Structural studies revealed that each small molecule interacted with the oxidized catalytic type 2 copper of AfNiR. Three small molecules (formate, acetate and nitrate) mimic the substrate by having at least two oxygen atoms for bidentate coordination to the type 2 copper atom. These three anions bound to the copper ion in the same asymmetric, bidentate manner as nitrite. Consistent with their weak inhibition of the enzyme ( K i >50 mM), the Cu-O distances in these AfNiR-inhibitor complexes were approximately 0.15 A longer than that observed in the AfNiR-nitrite complex. The binding mode of each inhibitor is determined in part by steric interactions with the side chain of active site residue Ile257. Moreover, the side chain of Asp98, a conserved residue that hydrogen bonds to type 2 copper-bound nitrite and nitric oxide, was either disordered or pointed away from the inhibitors. Acetate and formate inhibited AfNiR in a mixed fashion, consistent with the occurrence of second acetate binding site in the AfNiR-acetate complex that occludes access to the type 2 copper. A fourth small molecule, nitrous oxide, bound to the oxidized metal in a side-on fashion reminiscent of nitric oxide to the reduced copper. Nevertheless, nitrous oxide bound at a farther distance from the metal. The fifth small molecule, azide, inhibited the reduction of nitrite by AfNiR most strongly ( K ic = 2.0 +/- 0.1 mM). This ligand bound to the type 2 copper center end-on with a Cu-N c distance of approximately 2 A, and was the only inhibitor to form a hydrogen bond with Asp98. Overall, the data substantiate the roles of Asp98 and Ile257 in discriminating substrate from other small anions.

PubMed: 18358002
DOI: 10.1021/bi7020537

実験手法

X-RAY DIFFRACTION (1.5 Å)