2E5L

A snapshot of the 30S ribosomal subunit capturing mRNA via the Shine- Dalgarno interaction

2E5L の概要

• エントリーDOI: 10.2210/pdb2e5l/pdb
• 分子名称: 16S ribosomal RNA, 30S ribosomal protein S9, 30S ribosomal protein S10, ... (23 entities in total)
• 機能のキーワード: 30s ribosomal subunit, mrna capture, shine-dalgarno interaction, 5' untranslated region, translation initiation, structural genomics, nppsfa, national project on protein structural and functional analyses, riken structural genomics/proteomics initiative, rsgi, ribosome
• 由来する生物種: Thermus thermophilus; 詳細
• タンパク質・核酸の鎖数: 23
• 化学式量合計: 781798.51

構造登録者

Kaminishi, T.,Wilson, D.N.,Takemoto, C.,Harms, J.M.,Kawazoe, M.,Schluenzen, F.,Hanawa-Suetsugu, K.,Shirouzu, M.,Fucini, P.,Yokoyama, S.,RIKEN Structural Genomics/Proteomics Initiative (RSGI) (登録日: 2006-12-21, 公開日: 2007-05-15, 最終更新日: 2024-03-13)

主引用文献

Kaminishi, T.,Wilson, D.N.,Takemoto, C.,Harms, J.M.,Kawazoe, M.,Schluenzen, F.,Hanawa-Suetsugu, K.,Shirouzu, M.,Fucini, P.,Yokoyama, S.
A snapshot of the 30S ribosomal subunit capturing mRNA via the Shine-Dalgarno interaction
Structure, 15:289-297, 2007

PubMed Abstract: In the initiation phase of bacterial translation, the 30S ribosomal subunit captures mRNA in preparation for binding with initiator tRNA. The purine-rich Shine-Dalgarno (SD) sequence, in the 5' untranslated region of the mRNA, anchors the 30S subunit near the start codon, via base pairing with an anti-SD (aSD) sequence at the 3' terminus of 16S rRNA. Here, we present the 3.3 A crystal structure of the Thermus thermophilus 30S subunit bound with an mRNA mimic. The duplex formed by the SD and aSD sequences is snugly docked in a "chamber" between the head and platform domains, demonstrating how the 30S subunit captures and stabilizes the otherwise labile SD helix. This location of the SD helix is suitable for the placement of the start codon AUG in the immediate vicinity of the mRNA channel, in agreement with reported crosslinks between the second position of the start codon and G1530 of 16S rRNA.

PubMed: 17355865
DOI: 10.1016/j.str.2006.12.008

実験手法

X-RAY DIFFRACTION (3.3 Å)