2E5C

Crystal structure of Human NMPRTase complexed with 5'-phosphoribosyl-1'-pyrophosphate

2E5C の概要

• エントリーDOI: 10.2210/pdb2e5c/pdb
• 関連するPDBエントリー: 2E5B 2E5D
• 分子名称: Nicotinamide phosphoribosyltransferase, 1-O-pyrophosphono-5-O-phosphono-alpha-D-ribofuranose (3 entities in total)
• 機能のキーワード: nmprtase, pbef, visfatin, prtase, transferase
• 由来する生物種: Homo sapiens (human)
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 113537.76

構造登録者

Takahashi, R.,Nakamura, S.,Kobayashi, Y.,Ohkubo, T. (登録日: 2006-12-20, 公開日: 2007-12-25, 最終更新日: 2023-10-25)

主引用文献

Takahashi, R.,Nakamura, S.,Nakazawa, T.,Minoura, K.,Yoshida, T.,Nishi, Y.,Kobayashi, Y.,Ohkubo, T.
Structure and reaction mechanism of human nicotinamide phosphoribosyltransferase
J.Biochem., 147:95-107, 2010

PubMed Abstract: Nicotinamide (NM) phosphoribosyltransferase (NMPRTase) catalyzes the reaction of NM and 5'-phosphoribosyl-1'-pyrophosphate (PRPP) to form NM mononucleotide (NMN) and pyrophosphate (PPi) in the pathway of NAD-biosynthesis. Monitoring the (1)H and (31)P NMR spectra of the reaction mixture, we found that this reaction is reversible as dictated by the equilibrium constant K = [NMN][PPi]/([NM][PRPP]) = 0.14, which agreed well with the ratio of second-order rate constants for forward and backward reactions, K = 0.16. The crystal structures of this enzyme in the free form and bound to NM and PRPP at the resolution of 2.0-2.2 A were essentially identical to that of the complex with NMN, except for some variations that could facilitate the substitution reaction by fixing the nucleophile and the leaving group for the requisite inversion of configuration at the C1' carbon of the ribose ring. In the active site near the C1' atom of the bound PRPP or NMN, there was neither negatively charged group nor waterproof environment necessary to support the feasibility of a ribo-oxocarbocation intermediate inherent in the S(N)1 mechanism. The structures and catalytic mechanism thus revealed are also discussed in connection with the multiple biological functions of NMPRTase.

PubMed: 19819904
DOI: 10.1093/jb/mvp152

実験手法

X-RAY DIFFRACTION (2.2 Å)