2E30

Solution structure of the cytoplasmic region of Na+/H+ exchanger 1 complexed with essential cofactor calcineurin B homologous protein 1

2E30 の概要

• エントリーDOI: 10.2210/pdb2e30/pdb
• 分子名称: Calcium-binding protein p22, Sodium/hydrogen exchanger 1, CALCIUM ION (3 entities in total)
• 機能のキーワード: transporter, ef-hand, complex structure, metal binding protein-transport protein complex, metal binding protein/transport protein
• 由来する生物種: Homo sapiens (human); 詳細
• 細胞内の位置: Cytoplasm (By similarity): Q99653; Membrane; Multi-pass membrane protein: P19634
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 27583.04

構造登録者

Mishima, M.,Wakabayashi, S.,Kojima, C. (登録日: 2006-11-19, 公開日: 2006-12-19, 最終更新日: 2024-05-29)

主引用文献

Mishima, M.,Wakabayashi, S.,Kojima, C.
Solution structure of the cytoplasmic region of Na+/H+ exchanger 1 complexed with essential cofactor calcineurin B homologous protein 1
J.Biol.Chem., 282:2741-2751, 2007

PubMed Abstract: Na+/H+ exchanger 1 (NHE1) regulates intracellular pH, Na+ content, and cell volume. Calcineurin B homologous protein 1 (CHP1) serves as an essential cofactor that facilitates NHE1 exchange activity under physiological conditions by direct binding to the cytoplasmic juxtamembrane region of NHE1. Here we describe the solution structure of the cytoplasmic juxtamembrane region of NHE1 complexed with CHP1. The region of NHE1 forms an amphipathic helix, which is induced by CHP1 binding, and CHP1 possesses a large hydrophobic cleft formed by EF-hand helices. The apolar side of the NHE1 helix participates in extensive hydrophobic interactions with the cleft of CHP1. We suggest that helix formation of the cytoplasmic region of NHE1 by CHP1 is a prerequisite for generating the active form of NHE1. The molecular recognition detailed in this study also provides novel insight into the target binding mechanism of EF-hand proteins.

PubMed: 17050540
DOI: 10.1074/jbc.M604092200

実験手法

SOLUTION NMR