2E2S

Solution structure of agelenin, an insecticidal peptide from the venom of Agelena opulenta

2E2S の概要

• エントリーDOI: 10.2210/pdb2e2s/pdb
• 分子名称: Agelenin (1 entity in total)
• 機能のキーワード: cystine knot, presynaptic calcium channel inhibitor, toxin
• 由来する生物種: Allagelena opulenta
• 細胞内の位置: Secreted: P31328
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 3829.53

構造登録者

Yamaji, N. (登録日: 2006-11-17, 公開日: 2007-08-07, 最終更新日: 2024-10-30)

主引用文献

Yamaji, N.,Sugase, K.,Nakajima, T.,Miki, T.,Wakamori, M.,Mori, Y.,Iwashita, T.
Solution structure of agelenin, an insecticidal peptide isolated from the spider Agelena opulenta, and its structural similarities to insect-specific calcium channel inhibitors
Febs Lett., 581:3789-3794, 2007

PubMed Abstract: Agelenin, isolated from the Agelenidae spider Agelena opulenta, is a peptide composed of 35 amino acids. We determined the three-dimensional structure of agelenin using two-dimensional NMR spectroscopy. The structure is composed of a short antiparallel beta-sheet and four beta-turns, which are stabilized by three disulfide bonds. Agelenin has characteristic residues, Phe9, Ser28 and Arg33, which are arranged similarly to the pharmacophore of the insect channel inhibitor, omega-atracotoxin-Hv1a. These observations suggest that agelenin and omega-atracotoxin-Hv1a bind to insect calcium channels in a similar manner. We also suggest that another mode of action may operate in the channel inhibition by omega-agatoxin-IVA and omega-atracotoxin-Hv2a.

PubMed: 17644092
DOI: 10.1016/j.febslet.2007.06.077

実験手法

SOLUTION NMR