2E0Z

Crystal structure of virus-like particle from Pyrococcus furiosus

2E0Z の概要

• エントリーDOI: 10.2210/pdb2e0z/pdb
• 分子名称: Virus-like particle (1 entity in total)
• 機能のキーワード: virus-like particle, virus, bacteriophage, hk97, t4, virus like particle
• 由来する生物種: Pyrococcus furiosus
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 116549.29

構造登録者

Akita, F.,Chong, K.T.,Tanaka, H.,Yamashita, E.,Miyazaki, N.,Nakaishi, Y.,Namba, K.,Ono, Y.,Suzuki, M.,Tsukihara, T.,Nakagawa, A. (登録日: 2006-10-16, 公開日: 2007-04-17, 最終更新日: 2024-03-13)

主引用文献

Akita, F.,Chong, K.T.,Tanaka, H.,Yamashita, E.,Miyazaki, N.,Nakaishi, Y.,Suzuki, M.,Namba, K.,Ono, Y.,Tsukihara, T.,Nakagawa, A.
The Crystal Structure of a Virus-like Particle from the Hyperthermophilic Archaeon Pyrococcus furiosus Provides Insight into the Evolution of Viruses
J.Mol.Biol., 368:1469-1483, 2007

PubMed Abstract: Pyrococcus furiosus is a hyperthermophilic archaeal microorganism found near deep-sea thermal vents and its optimal growth temperature of 100 degrees C. Recently, a 38.8-kDa protein from P. furiosus DSM 3638 was isolated and characterized. Electron microscopy revealed that this protein aggregated as spheres of approximately 30 nm in diameter, which we designated P. furiosus virus-like particles (PfVs). X-ray crystallographic analysis at 3.6-A resolution revealed that each PfV consisted of 180 copies of the 38.8-kDa protein and retained T=3 icosahedral symmetry, as is often the case in spherical viruses. The total molecular mass of each particle was approximately 7 MDa. An examination of capsid structures suggested strong evolutionary links among PfV, tailed double-stranded DNA bacteriophages, and herpes viruses. The similar three-dimensional structures of the various coat proteins indicate that these viral capsids might have originated and evolved from a common ancestor. The structure of PfV provides a previously undescribed example of viral relationships across the three domains of life (Eukarya, Bacteria, and Archaea).

PubMed: 17397865
DOI: 10.1016/j.jmb.2007.02.075

実験手法

X-RAY DIFFRACTION (3.6 Å)