2DSK

Crystal structure of catalytic domain of hyperthermophilic chitinase from Pyrococcus furiosus

Summary for 2DSK

Entry DOI	10.2210/pdb2dsk/pdb
Descriptor	chitinase, GLYCEROL, SULFATE ION, ... (4 entities in total)
Functional Keywords	chitinase, catalytic domain, active domain, crystalline chitin, tim-barrel, hydrolase
Biological source	Pyrococcus furiosus
Total number of polymer chains	2
Total formula weight	70177.08
Authors	Nakamura, T.,Mine, S.,Hagihara, Y.,Ishikawa, K.,Uegaki, K. (deposition date: 2006-06-30, release date: 2007-02-06, Last modification date: 2024-03-13)
Primary citation	Nakamura, T.,Mine, S.,Hagihara, Y.,Ishikawa, K.,Uegaki, K. Structure of the catalytic domain of the hyperthermophilic chitinase from Pyrococcus furiosus ACTA CRYSTALLOGR.,SECT.F, 63:7-11, 2007 Cited by PubMed Abstract: The crystal structure of the catalytic domain of a chitinase from the hyperthermophilic archaeon Pyrococcus furiosus (AD2(PF-ChiA)) has been determined at 1.5 A resolution. This is the first structure of the catalytic domain of an archaeal chitinase. The overall structure of AD2(PF-ChiA) is a TIM-barrel fold with a tunnel-like active site that is a common feature of family 18 chitinases. Although the catalytic residues (Asp522, Asp524 and Glu526) are conserved, comparison of the conserved residues and structures with those of other homologous chitinases indicates that the catalytic mechanism of PF-ChiA is different from that of family 18 chitinases. PubMed: 17183162 DOI: 10.1107/S1744309106051773 PDB entries with the same primary citation
Experimental method	X-RAY DIFFRACTION (1.5 Å)

Structure validation