2DPK
The Crystal Structure of the Primary Ca2+ Sensor of the Na+/Ca2+ Exchanger
Summary for 2DPK
| Entry DOI | 10.2210/pdb2dpk/pdb |
| Descriptor | Sodium/calcium exchanger 1, CALCIUM ION, GUANIDINE, ... (5 entities in total) |
| Functional Keywords | calcium sensory domain, beta-sandwich, cis-proline, beta-bulge, metal transport |
| Biological source | Canis lupus familiaris (dog) |
| Cellular location | Cell membrane; Multi-pass membrane protein: P23685 |
| Total number of polymer chains | 1 |
| Total formula weight | 17149.94 |
| Authors | Abramson, J.,Sawaya, M. (deposition date: 2006-05-12, release date: 2006-06-13, Last modification date: 2024-10-23) |
| Primary citation | Nicoll, D.A.,Sawaya, M.,Kwon, S.,Cascio, D.,Philipson, K.D.,Abramson, J. The crystal structure of the primary Ca2+ sensor of the na+/ca2+ exchanger reveals a novel Ca2+ binding motif. J.Biol.Chem., 281:21577-21581, 2006 Cited by PubMed Abstract: The Na+/Ca2+ exchanger is a plasma membrane protein that regulates intracellular Ca2+ levels in cardiac myocytes. Transport activity is governed by Ca2+, and the primary Ca2+ sensor (CBD1) is located in a large cytoplasmic loop connecting two transmembrane helices. The binding of Ca2+ to the CBD1 sensory domain results in conformational changes that stimulate the exchanger to extrude Ca2+. Here, we present a crystal structure of CBD1 at 2.5A resolution, which reveals a novel Ca2+ binding site consisting of four Ca2+ ions arranged in a tight planar cluster. This intricate coordination pattern for a Ca2+ binding cluster is indicative of a highly sensitive Ca2+ sensor and may represent a general platform for Ca2+ sensing. PubMed: 16774926DOI: 10.1074/jbc.C600117200 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (2.5 Å) |
Structure validation
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