2DPK

The Crystal Structure of the Primary Ca2+ Sensor of the Na+/Ca2+ Exchanger

2DPK の概要

• エントリーDOI: 10.2210/pdb2dpk/pdb
• 分子名称: Sodium/calcium exchanger 1, CALCIUM ION, GUANIDINE, ... (5 entities in total)
• 機能のキーワード: calcium sensory domain, beta-sandwich, cis-proline, beta-bulge, metal transport
• 由来する生物種: Canis lupus familiaris (dog)
• 細胞内の位置: Cell membrane; Multi-pass membrane protein: P23685
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 17149.94

構造登録者

Abramson, J.,Sawaya, M. (登録日: 2006-05-12, 公開日: 2006-06-13, 最終更新日: 2024-10-23)

主引用文献

Nicoll, D.A.,Sawaya, M.,Kwon, S.,Cascio, D.,Philipson, K.D.,Abramson, J.
The crystal structure of the primary Ca2+ sensor of the na+/ca2+ exchanger reveals a novel Ca2+ binding motif.
J.Biol.Chem., 281:21577-21581, 2006

PubMed Abstract: The Na+/Ca2+ exchanger is a plasma membrane protein that regulates intracellular Ca2+ levels in cardiac myocytes. Transport activity is governed by Ca2+, and the primary Ca2+ sensor (CBD1) is located in a large cytoplasmic loop connecting two transmembrane helices. The binding of Ca2+ to the CBD1 sensory domain results in conformational changes that stimulate the exchanger to extrude Ca2+. Here, we present a crystal structure of CBD1 at 2.5A resolution, which reveals a novel Ca2+ binding site consisting of four Ca2+ ions arranged in a tight planar cluster. This intricate coordination pattern for a Ca2+ binding cluster is indicative of a highly sensitive Ca2+ sensor and may represent a general platform for Ca2+ sensing.

PubMed: 16774926
DOI: 10.1074/jbc.C600117200

実験手法

X-RAY DIFFRACTION (2.5 Å)