2DFL
Crystal structure of left-handed RadA filament
Summary for 2DFL
| Entry DOI | 10.2210/pdb2dfl/pdb |
| Related | 1PZN 1T4G |
| Descriptor | DNA repair and recombination protein radA (2 entities in total) |
| Functional Keywords | archaea, filament, left-handed, dna binding, recombination, molecular switch, reca, rad51, dmc1 |
| Biological source | Sulfolobus solfataricus |
| Total number of polymer chains | 1 |
| Total formula weight | 35914.03 |
| Authors | Chen, L.T.,Ko, T.P.,Wang, T.F.,Wang, A.H.J. (deposition date: 2006-03-02, release date: 2007-01-23, Last modification date: 2023-10-25) |
| Primary citation | Chen, L.T.,Ko, T.P.,Chang, Y.C.,Lin, K.A.,Chang, C.S.,Wang, A.H.J.,Wang, T.F. Crystal structure of the left-handed archaeal RadA helical filament: identification of a functional motif for controlling quaternary structures and enzymatic functions of RecA family proteins Nucleic Acids Res., 35:1787-1801, 2007 Cited by PubMed Abstract: The RecA family of proteins mediates homologous recombination, an evolutionarily conserved pathway that maintains genomic stability by protecting against DNA double strand breaks. RecA proteins are thought to facilitate DNA strand exchange reactions as closed-rings or as right-handed helical filaments. Here, we report the crystal structure of a left-handed Sulfolobus solfataricus RadA helical filament. Each protomer in this left-handed filament is linked to its neighbour via interactions of a beta-strand polymerization motif with the neighbouring ATPase domain. Immediately following the polymerization motif, we identified an evolutionarily conserved hinge region (a subunit rotation motif) in which a 360 degrees clockwise axial rotation accompanies stepwise structural transitions from a closed ring to the AMP-PNP right-handed filament, then to an overwound right-handed filament and finally to the left-handed filament. Additional structural and functional analyses of wild-type and mutant proteins confirmed that the subunit rotation motif is crucial for enzymatic functions of RecA family proteins. These observations support the hypothesis that RecA family protein filaments may function as rotary motors. PubMed: 17329376DOI: 10.1093/nar/gkl1131 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (2.9 Å) |
Structure validation
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