2DFL

Crystal structure of left-handed RadA filament

2DFL の概要

• エントリーDOI: 10.2210/pdb2dfl/pdb
• 関連するPDBエントリー: 1PZN 1T4G
• 分子名称: DNA repair and recombination protein radA (2 entities in total)
• 機能のキーワード: archaea, filament, left-handed, dna binding, recombination, molecular switch, reca, rad51, dmc1
• 由来する生物種: Sulfolobus solfataricus
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 35914.03

構造登録者

Chen, L.T.,Ko, T.P.,Wang, T.F.,Wang, A.H.J. (登録日: 2006-03-02, 公開日: 2007-01-23, 最終更新日: 2023-10-25)

主引用文献

Chen, L.T.,Ko, T.P.,Chang, Y.C.,Lin, K.A.,Chang, C.S.,Wang, A.H.J.,Wang, T.F.
Crystal structure of the left-handed archaeal RadA helical filament: identification of a functional motif for controlling quaternary structures and enzymatic functions of RecA family proteins
Nucleic Acids Res., 35:1787-1801, 2007

PubMed Abstract: The RecA family of proteins mediates homologous recombination, an evolutionarily conserved pathway that maintains genomic stability by protecting against DNA double strand breaks. RecA proteins are thought to facilitate DNA strand exchange reactions as closed-rings or as right-handed helical filaments. Here, we report the crystal structure of a left-handed Sulfolobus solfataricus RadA helical filament. Each protomer in this left-handed filament is linked to its neighbour via interactions of a beta-strand polymerization motif with the neighbouring ATPase domain. Immediately following the polymerization motif, we identified an evolutionarily conserved hinge region (a subunit rotation motif) in which a 360 degrees clockwise axial rotation accompanies stepwise structural transitions from a closed ring to the AMP-PNP right-handed filament, then to an overwound right-handed filament and finally to the left-handed filament. Additional structural and functional analyses of wild-type and mutant proteins confirmed that the subunit rotation motif is crucial for enzymatic functions of RecA family proteins. These observations support the hypothesis that RecA family protein filaments may function as rotary motors.

PubMed: 17329376
DOI: 10.1093/nar/gkl1131

実験手法

X-RAY DIFFRACTION (2.9 Å)