2DFC

Xylanase II from Tricoderma reesei at 293K

2DFC の概要

• エントリーDOI: 10.2210/pdb2dfc/pdb
• 関連するPDBエントリー: 2DFB
• 分子名称: Endo-1,4-beta-xylanase 2, IODIDE ION (3 entities in total)
• 機能のキーワード: bent beta sheet, hydrolase
• 由来する生物種: Hypocrea jecorina
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 20965.34

構造登録者

Harata, K.,Akiba, T. (登録日: 2006-02-28, 公開日: 2006-07-11, 最終更新日: 2024-10-23)

主引用文献

Watanabe, N.,Akiba, T.,Kanai, R.,Harata, K.
Structure of an orthorhombic form of xylanase II from Trichoderma reesei and analysis of thermal displacement.
ACTA CRYSTALLOGR.,SECT.D, 62:784-792, 2006

PubMed Abstract: An orthorhombic crystal of xylanase II from Trichoderma reesei was grown in the presence of sodium iodide. Crystal structures at atomic resolution were determined at 100 and 293 K. Protein molecules were aligned along a crystallographic twofold screw axis, forming a helically extended polymer-like chain mediated by an iodide ion. The iodide ion connected main-chain peptide groups between two adjacent molecules by an N-H...I-...H-N hydrogen-bond bridge, thus contributing to regulation of the molecular arrangement and suppression of the rigid-body motion in the crystal with high diffraction quality. The structure at 293 K showed considerable thermal motion in the loop regions connecting the beta-strands that form the active-site cleft. TLS model analysis of the thermal motion and a comparison between this structure and that at 100 K suggest that the fluctuation of these loop regions is attributable to the hinge-like movement of the beta-strands.

PubMed: 16790934
DOI: 10.1107/S0907444906017379

実験手法

X-RAY DIFFRACTION (1.19 Å)