2DCE
Solution structure of the SWIRM domain of human KIAA1915 protein
Summary for 2DCE
| Entry DOI | 10.2210/pdb2dce/pdb |
| Descriptor | KIAA1915 protein (1 entity in total) |
| Functional Keywords | swirm domain, kiaa1915, structural genomics, nppsfa, national project on protein structural and functional analyses, riken structural genomics/proteomics initiative, rsgi, unknown function |
| Biological source | Homo sapiens (human) |
| Cellular location | Nucleus: Q5VVJ2 |
| Total number of polymer chains | 1 |
| Total formula weight | 12829.41 |
| Authors | Yoneyama, M.,Tochio, N.,Umehara, T.,Koshiba, S.,Inoue, M.,Tanaka, A.,Kigawa, T.,Yokoyama, S.,RIKEN Structural Genomics/Proteomics Initiative (RSGI) (deposition date: 2006-01-06, release date: 2006-07-06, Last modification date: 2024-05-29) |
| Primary citation | Yoneyama, M.,Tochio, N.,Umehara, T.,Koshiba, S.,Inoue, M.,Yabuki, T.,Aoki, M.,Seki, E.,Matsuda, T.,Watanabe, S.,Tomo, Y.,Nishimura, Y.,Harada, T.,Terada, T.,Shirouzu, M.,Hayashizaki, Y.,Ohara, O.,Tanaka, A.,Kigawa, T.,Yokoyama, S. Structural and Functional Differences of SWIRM Domain Subtypes J.Mol.Biol., 369:222-238, 2007 Cited by PubMed Abstract: SWIRM is a conserved domain found in several chromatin-associated proteins. Based on their sequences, the SWIRM family members can be classified into three subfamilies, which are represented by Swi3, LSD1, and Ada2. Here we report the SWIRM structure of human MYb-like, Swirm and Mpn domain-containing protein-1 (MYSM1). The MYSM1 SWIRM structure forms a compact HTH-related fold comprising five alpha-helices, which best resembles the Swi3 SWIRM structure, among the known SWIRM structures. The MYSM1 and Swi3 SWIRM structures are more similar to the LSD1 structure than the Ada2alpha structure. The SWIRM domains of MYSM1 and LSD1 lacked DNA binding activity, while those of Ada2alpha and the human Swi3 counterpart, SMARCC2, bound DNA. The dissimilarity in the DNA-binding ability of the MYSM1 and SMARCC2 SWIRM domains might be due to a couple of amino acid differences in the last helix. These results indicate that the SWIRM family has indeed diverged into three structural subfamilies (Swi3/MYSM1, LSD1, and Ada2 types), and that the Swi3/MYSM1-type subfamily has further diverged into two functionally distinct groups. We also solved the structure of the SANT domain of MYSM1, and demonstrated that it bound DNA with a similar mode to that of the c-Myb DNA-binding domain. PubMed: 17428495DOI: 10.1016/j.jmb.2007.03.027 PDB entries with the same primary citation |
| Experimental method | SOLUTION NMR |
Structure validation
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