2DBE

CRYSTAL STRUCTURE OF A BERENIL-DODECANUCLEOTIDE COMPLEX: THE ROLE OF WATER IN SEQUENCE-SPECIFIC LIGAND BINDING

2DBE の概要

• エントリーDOI: 10.2210/pdb2dbe/pdb
• 分子名称: DNA (5'-D(*CP*GP*CP*GP*AP*AP*TP*TP*CP*GP*CP*G)-3'), BERENIL (3 entities in total)
• 機能のキーワード: b-dna, double helix, complexed with drug, dna
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 7608.10

構造登録者

Brown, D.G.,Sanderson, M.R.,Skelly, J.V.,Jenkins, T.C.,Brown, T.,Garman, E.,Stuart, D.I.,Neidle, S. (登録日: 1990-03-19, 公開日: 1991-07-15, 最終更新日: 2024-02-14)

主引用文献

Brown, D.G.,Sanderson, M.R.,Skelly, J.V.,Jenkins, T.C.,Brown, T.,Garman, E.,Stuart, D.I.,Neidle, S.
Crystal structure of a berenil-dodecanucleotide complex: the role of water in sequence-specific ligand binding.
EMBO J., 9:1329-1334, 1990

PubMed Abstract: The three-dimensional structure of a complex between the dodecanucleotide d(CGCGAATTCGCG) and the anti-trypanocidal drug berenil, has been determined to a resolution of 2.5 A. The structure has been solved by molecular replacement and refined to an R factor of 0.177. A total of 49 water molecules have been located. The drug is bound at the 5'-AAT-3' region of the oligonucleotide. At one end of the drug the amidinium group is in hydrogen-bonded contact with N3 of the adenine base complementary to the thymine of the AAT. The other amidinium group does not make direct interactions with the DNA. Instead, a water molecule mediates between them. This is in hydrogen-bonded contact with an amidinium nitrogen atom, N3 of the 5' end adenine base and the ring oxygen atom of an adjacent deoxyribose. Molecular mechanics calculations have been performed on this complex, with the drug at various positions along the sequence. These show that the observed position is only 0.8 kcal/mol higher in energy than the best position. It is suggested that there is a broad energy well in the AATT region for this drug, and that water molecules as well as the neighbouring sequence, will determine precise positioning. More general aspects of minor groove binding are discussed.

PubMed: 2323343

実験手法

X-RAY DIFFRACTION (2.5 Å)