2D94

THE CONFORMATION OF THE DNA DOUBLE HELIX IN THE CRYSTAL IS DEPENDENT ON ITS ENVIRONMENT

2D94 の概要

• エントリーDOI: 10.2210/pdb2d94/pdb
• 分子名称: DNA (5'-D(*GP*GP*GP*CP*GP*CP*CP*C)-3') (2 entities in total)
• 機能のキーワード: a-dna, double helix, dna
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 2428.59

構造登録者

Shakked, Z.,Guerstein-Guzikevich, G.,Eisenstein, M.,Frolow, F.,Rabinovich, D. (登録日: 1993-07-13, 公開日: 1994-01-15, 最終更新日: 2024-02-14)

主引用文献

Shakked, Z.,Guerstein-Guzikevich, G.,Eisenstein, M.,Frolow, F.,Rabinovich, D.
The conformation of the DNA double helix in the crystal is dependent on its environment.
Nature, 342:456-460, 1989

PubMed Abstract: Studies of the crystal structures of more than 30 synthetic DNA fragments have provided structural information about three basic forms of the double helix: A-, B- and Z-form DNA. These studies have demonstrated that the DNA double helix adopts a highly variable structure which is related to its base sequence. The extent to which such observed structures are influenced by the crystalline environment can be found by studying the same molecule in different crystalline forms. We have recently crystallized one particular oligomer in various crystal forms. Here we report the results of structural analyses of the different crystal structures and demonstrate that the DNA double helix can adopt a range of conformations in the crystalline state depending on hydration, molecular packing and temperature. These results have implications on our understanding of the influence of the environment on DNA structure, and on the modes of DNA recognition by proteins.

PubMed: 2586615
DOI: 10.1038/342456a0

実験手法

X-RAY DIFFRACTION (1.7 Å)